世界中医药Issue(12)：1636-1639,4.DOI:10.3969/j.issn.1673-7202.2014.12.020
新加良附方联合5-Fu 对荷人胃癌裸鼠血清VEGF、bFGF 水平的影响
The Effects of Modified Liang-Fu Formula Combined with 5-Fu on VEGF,bFGF Expression of Human Gastric Cancer Xenografts in Nude Mice
摘要
Abstract
Objective:To observe the effect of Modified Liang-fu Formula (MLFF)combined with 5-Fu on VEGF and bFGF expression in the serum of human gastric cancer xenografted nude mice.Methods:The animal model of SGC-7901 xenografted nude mice was estab-lished,and all the mice were randomly divided into 6 groups;namely model control group (the same amount of normal sterile saline),5-Fu group (17 mg·d -1 ·kg -1 ),MLFF high-dose group (10 g·d -1 ·kg -1 ),MLFF medium-dose group (5 g·d -1 ·kg -1 ),MLFF low-dose group (2.5 g·d -1 ·kg -1 )and drug combination group (5 g·d -1 ·kg -1 MLFF combined with 17 mg·d -1 ·kg -1 5-Fu). After 10 days of continuous treatment,eyeball blood was withdrew,the mice were killed,and the expression of VEGF and bFGF in the se-rum were detected using ELISA method.Results:Compared to model control group,the expression levels of VEGF in nude mice serum were significantly reduced in 5-Fu group,MLFF high-dose group,MLFF medium-dose group,MLFF low-dose group and drug combination group,respectively (P <0.01),while MLFF reduced VEGF expression in a dose-dependent manner.The combination group showed a lower level of VEGF compared to 5-Fu group and MLFF medium-dose group,but there was no significant difference (P <0.05).Mean-while,compared to model control group,the expression level of bFGF in nude mice serum was significantly decreased in 5-Fu group, MLFF high-dose group,MLFF medium-dose group,MLFF low-dose group and drug combination group,respectively (P <0.01).Conclu-sion:MLFF can down-regulate the expression of VEGF,bFGF in the serum of human gastric cancer xenografted nude mice.It indicates that MLFF has the potential to serve as anti-angiogenesis agent for gastric cancer.关键词
新加良附方/新加良附方联合 5-Fu/VEGF/bFGFKey words
Modified Liang-fu formula/Modified Liang-fu formula combined with 5-Fu/VEGF/bFGF分类
医药卫生引用本文复制引用
侯丽,许晶,王婧,郭晓青,宋延平..新加良附方联合5-Fu 对荷人胃癌裸鼠血清VEGF、bFGF 水平的影响[J].世界中医药,2014,(12):1636-1639,4.基金项目
北京中医药大学科学科研基地建设项目北京市重点实验室（中医内科学）---新加良附方抗胃癌效应机制研究 （）
