摘要
Abstract
Objective:To establish a high performance liquid phase method to measure the content of chlorogenic acid of Flos Lonicerae in Ganmao granules,and to ensure that the results are accurate and reliable by systematic methodology.Methods:The chromatographic column:Agilent TC-C18 column (4.6 ×150 mm,5 μm,Agilent,American),a mobile phase of methanol:-0. 5%phosphoric acid solution (15:85),the detection wavelength was 327 nm.Results:The chlorogenic acid in 25ug mL-1 -800ug・ mL-1 linear relationship was good,the linear regression equation was Y =27.88X+2.128,correlation coefficient was 0.999 8 (n =6);low,high concentrations of RSD precision,values were 0.59%,0.58%,0.44%;repetitive RSD 0.26%;low,recover-ies.The high concentration were respectively 99.24%,99.43%,99.75%;the test solution was placed at room temperature for 8 h,and RSD was 0.29%.Conclusion:This method for determination of the content of chlorogenic acid in Flos Lonicerae cold gran-ule is accurate and reliable with simple operation and short time.It can be used for the determination of chlorogenic acid in Flos Lonicerae in Ganmao granules.关键词
高效液相色谱法/绿原酸/银花感冒冲剂/含量测定Key words
HPLC/Chlorogenic acid/Yinhuaganmao granules/Content determination分类
医药卫生
