世界中医药Issue(2)：162-168,173,8.DOI:10.3969/j.issn.1673-7202.2015.02.004
基于方证相关探讨茵陈蒿汤调控库普弗细胞功能及MAPK通路抗肝纤维化的作用机制
Antifibrotic mechanism of action based on the corresponding on Traditional Chinese Medicine formula-syndrome to explore Yin-Chen-Hao Decoction regulation of Kupffer cell function and MAPK pathways
摘要
Abstract
Objective:Our previous study showed significantly efficacy of series formula of Yin-chen-hao Decoction (YCHD)on fibrosis models in rats induced dimethynitrosamine (DMN).From the perspective of formula-efficacy-syndrome,we proposed a hy-pothesis ‘corresponding permit on formula-syndrome,gene regulation of formula on suffering body follow‘error free repair’princi-ple’and explore the mechanism of action of YCHD.Methods:Liver fibrosis was induced in rats with DMN.YCHD was adminis-trated intragastrically for 2 weeks for rats.At the end of the fourth week,all of the animals were sacrificed and their liver samples were collected for histology and gene chip analyses.Results:Compared with the normal group,the blood serum ALT level of the rats was remarkably increased (P<0.01 )four weeks after modeling;the inflammatory cells of hepatic tissue was remarkably infil-trated according to pathological observance,the deposition of collagen was remarkable (P<0.01 ).There were remarkable up-reg-ulation of the expression of the genes including Interleukin 1 beta (IL-1 b),Cd68,Tumor necrosis factor receptor superfamily, member 1 4 (Tnfrsf1 4),Tumor necrosis factor receptor superfamily,member 9 (Tnfrsf9),Fas,Cd1 4,Connective tissue growth factor (Ctgf),Collagen,type I,alpha 2(Col1 a2),Insulin-like growth factor binding protein,acid labile subunit(Igfals),Insu-lin-like growth factor 1 (Igf1 ),Insulin-like growth factor binding protein 1 (Igfbp1 ),Matrix metallopeptidase 1 2(Mmp1 2),Matrix metallopeptidase 2(Mmp2),Matrix metallopeptidase 23(Mmp23),Chemokine ligand 21 (Ccl21 )and Protein kinase C,beta (Prkcb)in hepatic tissue,and mitogen-activated protein kinase (MAPK)pathway was activated.Yin-Chen-Hao Decoction signif-icantly reduced the level of blood serum ALT inducted by DMN,restrained the infiltration and necrosis of the inflammatory cells of hepatic tissue,inhibited collagen deposit,decreased the expression of the genes including IL-1 b,Cd68,Tnfrsf1 4,Tnfrsf9,Fas, Cd1 4,Ctgf,Col1 a2,Igfals,Igf1 ,Igfbp1 ,Mmp1 2,Mmp2,Mmp23,Ccl21 and Prkcb,and restrained the activation of MAPK pathway.Conclusion:The hypothesis ‘corresponding permit on formula-syndrome,gene regulation of formula on suffering body follow‘error free repair’principle’was verified.The MOA of anti-fibrotic of Yin-Chen-Hao Decoction was regulation of the KCs action and MAPK pathway.关键词
中医方证相关/茵陈蒿汤/肝纤维化/无差错修复原则/基因分析Key words
Corresponding on Traditional Chinese medicine formula-syndrome/Hepatic fibrosis/Yin-Chen-hao Decoction/‘Er-ror free repair’principle/Gene analysis分类
医药卫生引用本文复制引用
曹红燕,边艳琴,武超,李建缘,刘平,孙明瑜..基于方证相关探讨茵陈蒿汤调控库普弗细胞功能及MAPK通路抗肝纤维化的作用机制[J].世界中医药,2015,(2):162-168,173,8.基金项目
国家自然科学基金项目（编号81273729，30701070）；上海中医药大学首届杏林学者、上海市重点科技攻关项目（编号11 DZ1971702）；上海高校创新团队建设项目、国家中医药管理局中医肝胆病重点学科、慢性肝病虚损重点研究室和上海市中医临床重点实验室资助 （）
