色谱Issue(5)：447-451,5.DOI:10.3724/SP.J.1123.2013.12040
混合模式毛细管聚合物整体柱的制备及应用
Preparation and application of mixed-mode capillary polymer monolithic column
摘要
Abstract
Based on the diversity of the retention mechanism ,mixed-mode capillary monolithic columns have broad application prospect. In this work,a novel capillary polymer monolithic column was prepared with [ 2-( methacryloyloxy )ethyl ]dimethyl-( 3-sulfopropyl )ammonium hydroxide( SPE)as monomer,ethylene dimethacrylate( EDMA)as crosslinking agent,azobi-sisobutyronitrile( AIBN ) as initiator and butylalcohol/1,4-butanediol/water as ternary poro-gens. Under the optimized reaction conditions including the proportion of monomer and poro-gens,amount of initiator,reaction temperature and polymerization time,the monolithic col-umn showed good mechanical strength up to 10 MPa,high permeability of 2. 17 × 10-14 m 2 and good repeatability. The peak area RSDs of column-to-column and batch-to-batch reproducibility were 1. 0% and 4. 6%,respectively. Finally,the capillary monolithic column was evaluated with polar and non-polar test mixtures. It showed hydrophilic interaction under high organic phase while hydrophobic interaction under low organic phase,indicating it a mixed-mode monolithic column.关键词
毛细管高效液相色谱/毛细管整体柱/制备/混合模式Key words
capillary high performance liquid chromatography/capillary monolithic column/preparation/mixed-mode分类
化学化工引用本文复制引用
窦阿波,邹存杰,单圆鸿,石先哲,许国旺..混合模式毛细管聚合物整体柱的制备及应用[J].色谱,2014,(5):447-451,5.基金项目
国家自然科学基金项目（21275141）． （）
