色谱Issue(5)：524-528,5.DOI:10.3724/SP.J.1123.2014.01008
超高效液相色谱-串联质谱法测定动物源性食品中的磺胺增效剂
Determination of sulfonamide potentiators in animal origin foods by ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method for the determination of sulfonamide potentiators,trimethoprim( TMP), diaveridine(DVD)and ormetoprin(OMP),in different animal origin food matrices(including chicken muscle,fish muscle,chicken liver,egg and milk)has been developed by ultra perform-ance liquid chromatography-tandem mass spectrometry( UPLC-MS/MS). The sample was extrac-ted by formic acid-acetonitrile(1:9,v/v),cleaned-up by hexane,separated on an Acquity UPLC BEH C18 column(50 mm×2.1 mm,1.7 μm)with gradient elution. The determination was car-ried out with electrospray ion source under the positive mode and multiple reaction monitoring ( MRM)mode. The extraction recoveries of three extraction solvents were observed. The purifica-tion condition and concentration condition were optimized. In addition,the mobile phase,col-umn temperature and solid phase extraction column were studied. The calibration curves showed a good linearity in the range of 1. 25-30. 0 μg/L,and the correlation coefficients( r)were higher than 0. 99. The limits of quantification( LOQ,S/N=10)of the three potentiators were 5. 0 μg/kg. At the spiked levels of 5. 0,10. 0 and 20. 0 μg/kg,the recoveries of the three potentiators were ranged from 61. 2% to 108. 5%,and the relative standard deviations( RSD,n=6)ranged from 1. 1% to 9. 8%. The results indicate that the method is simple,rapid,sensitive and suitable for the qualitative and quantitative analysis of the three potentiators in multiple matrices.关键词
超高效液相色谱-串联质谱/磺胺增效剂/动物源性食品Key words
ultra performance liquid chromatography-tandem mass spectrometry( UPLC-MS/MS)/sulfonamide potentiators/animal origin foods分类
化学化工引用本文复制引用
高洋洋,张朝晖,刘鑫,卢晓宇,严华,何悦,杨大进,云环..超高效液相色谱-串联质谱法测定动物源性食品中的磺胺增效剂[J].色谱,2014,(5):524-528,5.基金项目
科技部质检公益行业科研专项（201210029）；国家高技术研究发展计划（“863”计划）项目（2012AA101603）． （）
