色谱Issue(6)：600-603,4.DOI:10.3724/SP.J.1123.2014.03031
两次双水相萃取结合高效液相色谱法选择性分离唾液蛋白质的初步研究
Preliminary study of two steps aqueous two-phase extraction combined with high performance liquid chromatography for saliva protein selective separation
摘要
Abstract
Saliva protein separation by one-step aqueous two-phase extraction( ATPE)in the system of 15%(mass ratio if not specified)PEG-4000 / 8% NaH 2 PO 4 was presented. The proteins extracted from the top and bottom phases were separated and characterized by high performance liquid chromatography( HPLC)respec-tively. Under the optimized gradient elution procedure,more saliva protein peaks in the two phases were shown. Saliva protein peaks in 50 min gradient were divided into ten specific groups which were classified into six sections based on the protein distribution in the top and bottom phases. Then,the top or bottom phase was performed the second step ATPE. Possible reasons for protein partition and retention difference are discussed briefly. Results show that some proteins can be separated completely in one-step ATPE,while some can be sep-arated in the following two-step ATPE. The combination of two steps ATPE and HPLC provides a new way to achieve selective separation.关键词
双水相萃取/两次萃取/高效液相色谱法/唾液蛋白Key words
aqueous two-phase extraction(ATPE)/two-step extraction/high performance liquid chromatog-raphy(HPLC)/saliva protein分类
化学化工引用本文复制引用
赵新颖,屈锋,覃浩,罗爱芹..两次双水相萃取结合高效液相色谱法选择性分离唾液蛋白质的初步研究[J].色谱,2014,(6):600-603,4.基金项目
National Basic Research Program of China（973 Program）（2012CB910603）；National Natural Science Foun-dation of China（21175011，21375008）． （）
