色谱Issue(6)：658-661,4.DOI:10.3724/SP.J.1123.2014.01023
气相色谱-三重四极杆串联质谱稳定同位素稀释法测定被动吸烟儿童尿液中的可丁宁
Determination of urinary cotinine of children exposed to passive smoking by stable isotope dilution gas chromatography-triple quadrupole mass spectrometry
摘要
Abstract
An analytical method for the determination of urinary cotinine of children exposed to passive smoking was established based on stable isotope dilution by gas chromatography-triple quadrupole mass spectrometry( GC-MS / MS). The samples were extracted and purified with chloroform. The extracts were determined by GC-MS / MS in multiple reaction monitoring (MRM)mode. The cotinine-d 3 as an isotope internal standard was applied to quantify and con-firm the urinary cotinine of children exposed to passive smoking. The method had a good linear-ity from 0. 1 μg / L to 10 μg / L with the correlation coefficient(r)﹥0. 998. The recoveries of the cotinine in blank urine were from 79. 2% to 112. 8% at spiked levels of 0. 1,1. 0 and 10 μg / L, with relative standard deviations(RSDs)from 2. 1% to 5. 8% . The limit of quantification(LOQ) of the method was 0. 1 μg / L. The developed method is accurate,sensitive,rapid and can be applied to detect urinary cotinine of children exposed to passive smoking at home.关键词
气相色谱-串联质谱/同位素稀释法/可丁宁/尿液/儿童/被动吸烟Key words
gas chromatography-tandem mass spectrometry( GC-MS/MS)/isotope dilution/cotinine/urine/children/passive smoking分类
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王芸,黄志强,叶英,张莹,肖水源..气相色谱-三重四极杆串联质谱稳定同位素稀释法测定被动吸烟儿童尿液中的可丁宁[J].色谱,2014,(6):658-661,4.基金项目
国家自然科学基金项目（81273089）；中南大学博士生创新基金项目（2013zzts076）． （）
