色谱Issue(7)：702-706,5.DOI:10.3724/SP.J.1123.2014.03002
固相萃取-超高效液相色谱-串联质谱法同时测定猪肉中20种精神药物残留
Simultaneous determination of twenty psychoactive drugs in pork by solid phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method for the simultaneous determination of 20 psychoactive drugs in pork by solid phase extraction coupled with ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry( UPLC-ESI-MS/MS)was established. The samples were extracted with alkalified acetonitrile,and then cleaned up using solid phase extraction with an Oasis MCX column. The UPLC separation was performed on a C18 column( 50 mm × 2. 1 mm, 1. 7 μm)using a gradient elution with the mobile phases of 0. 1%( v/v)formic acid in water and acetonitrile. The ESI-MS/MS detection was achieved in positive mode under multiple reac-tion monitoring( MRM)mode. The calibration curves showed good linearity in the range of 5-100 μg/L with the correlation coefficients more than 0. 99 for the 20 psychoactive drugs. The limits of quantification( LOQ,S/N≥10)for the 20 psychoactive drugs were 5 μg/kg. The aver-age recoveries of the 20 psychoactive drugs spiked in blank pork at three levels of 5,10 and 50μg/kg were 66. 8%-97. 2% with the relative standard deviations from 4. 2% to 12. 4%. The meth-od is suitable for the simultaneous determination of the 20 psychoactive drugs in pork with the characteristics of easy operation,high accuracy and precision.关键词
固相萃取/超高效液相色谱-电喷雾串联质谱/精神药物/猪肉Key words
solid phase extraction( SPE )/ultra performance liquid chromatography-electro-spary ionization tandem mass spectrometry( UPLC-ESI-MS/MS)/psychoactive drugs/pork分类
化学化工引用本文复制引用
孙婷,王鹭骁,曾三妹,吴敏,严丽娟,张峰,黄志强..固相萃取-超高效液相色谱-串联质谱法同时测定猪肉中20种精神药物残留[J].色谱,2014,(7):702-706,5.基金项目
国家科技支撑计划项目（2009BADB9B02，2012BAK08B01）；国家重大科学仪器设备开发专项项目（2012YQ14000806）． （）
