色谱Issue(7)：707-716,10.DOI:10.3724/SP.J.1123.2014.03003
QuEChERS-高效液相色谱-串联质谱法同时测定水果中21种植物生长调节剂的残留量
Determination of 21 plant growth regulator residues in fruits by QuEChERS-high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method for the simultaneous detection of 21 plant growth regulators in fruits by QuEChERS-high performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/MS)was developed. The samples were initially extracted with acetonitrile containing 1%( v/v) acetic acid,followed by clean-up using the powder of magnesium sulfate and C18. The resulting samples were separated on a C18 column,and detected under positive and negative multiple reactions monitoring( MRM ) mode through polarity switching between time segments. The matrix-matched external standard calibration curves were used for quantitative analysis. The linearities of chlormequat chloride,mepiquat chloride,choline chloride,cyclanilide,forchlor-fenuron,thidiazuron,inabenfide,paclobutrazol,uniconazole and triapenthenol were in the concentration range of 0. 1-500 μg/L,daminozide and 6-benzylaminopurine in the concentra-tion range of 1. 0-500 μg/L,2,3,5-triiodobenzoic acid,2,4-D,cloprop,4-chlorophenoxyacetic acid(4-CPA)and trinexapac-ethyl in the concentration range of 2. 0-1 000 μg/L,abscisic acid (ABA),gibberellic acid(GA3),1-naphthaleneacetic acid(NAA)and indol-3-ylacetic acid (IAA)in the concentration range of 10-1 000 μg/L,with the correlation coefficients higher than 0. 990. The limits of detection and the limits of quantification of the method were 0. 020-6. 0 μg/kg and 0. 10-15. 0 μg/kg,respectively. For all the samples,the average spiked recover-ies ranged from 73. 0% to 111. 0%,and the relative standard deviations( RSDs,n=6)were in the range of 3. 0%-17. 2%. The method is quick,easy,effective,sensitive and accurate,and can meet the requirements for the determination of the 21 plant growth regulator residues in fruits.关键词
QuEChERS/高效液相色谱-串联质谱法/植物生长调节剂/水果Key words
QuEChERS/high performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/MS)/plant growth regulators/fruits分类
化学化工引用本文复制引用
黄何何,张缙,徐敦明,周昱,罗佳,吕美玲,陈树宾,王连珠..QuEChERS-高效液相色谱-串联质谱法同时测定水果中21种植物生长调节剂的残留量[J].色谱,2014,(7):707-716,10.基金项目
福建省自然科学基金项目（2013J01031）；浙江省公益性技术应用研究计划项目（2012C37074）． （）
