色谱Issue(11)：1172-1180,9.DOI:10.3724/SP.J.1123.2014.07038
液相色谱-二极管阵列检测／质谱联用在活性染料结构鉴定中的应用
Structure identification of reactive dyes by high performance liquid chromatography-photodiode array detection/mass spectrometry
付新梅 1张蓉 1吴祖望1
作者信息
- 1. 大连理工大学，精细化工国家重点实验室，辽宁 大连 116024
- 折叠
摘要
Abstract
Based on years of experience on analyzing reactive dyes by high performance liquid chromatography-photodiode array detection / mass spectrometry( HPLC-DAD / MS),the essen-tial applications for current structure identification of the commercial reactive dyes are summa-rized in three aspects. Commercial dyes always include several kinds of components. They are assistants for increasing solubility of synthetic dyes,multi-color synthetic dyes and their by-products derived from synthesis and storage. HPLC-DAD / MS technique can separate most of the components,and provide UV-Vis spectra and mass spectra individually. The separation and analysis can be achieved only through one injection without pre-purification of commercial dyes. Compared with traditional analytical methods,HPLC-DAD / MS technique reduces greatly not only the workload of analysis but also provides fully the actual compositions of the dyestuff. The reactive groups can react with ethanolamine or diethanolamine under moderate condition without structure destruction of dyes. The molecular masses before and after derivatization can be determined by MS,and then the type and number of reactive groups can be ascertained by so called“mass difference by derivative method”. The chromophore of the dye can be identi-fied by full spectrum scanning function of DAD. HPLC-DAD / MS analytical technique is very special and useful for the structure analysis of multi-component mixed commercial dyes. As examples,the identifications of three multi-component mixed structures of commercial dyes (Reactive Gray,Reactive Supra Black and Reactive Marine Blue)were given.关键词
液相色谱-二极管阵列检测/质谱/电喷雾质谱/结构鉴定/活性染料Key words
high performance liquid chromatography-photodiode array detection/mass spec-trometry(HPLC-DAD/MS)/electrospray mass spectrometry/structure identification/reactive dyes分类
化学化工引用本文复制引用
付新梅,张蓉,吴祖望..液相色谱-二极管阵列检测／质谱联用在活性染料结构鉴定中的应用[J].色谱,2014,(11):1172-1180,9.
