色谱Issue(11)：1187-1196,10.DOI:10.3724/SP.J.1123.2014.06015
前体离子扫描法在快速筛查中药及保健食品中非法添加的5型磷酸二酯酶抑制剂及其未知衍生物中的应用
Application of precursor ion scanning method in rapid screening of illegally added phosphodiesterase-5 inhibitors and their unknown derivatives in Chinese traditional patent medicines and health foods
摘要
Abstract
The compounds with similar structure often have similar pharmacological activities. So it is a trend for illegal addition that new derivatives of effective drugs are synthesized to avoid the statutory test. This bring challenges to crack down on illegal addition behavior, however,modified derivatives usually have similar product ions,which allow for precursor ion scanning. In this work,precursor ion scanning mode of a triple quadrupole mass spectrometer was first applied to screen illegally added drugs in complex matrix such as Chinese traditional patent medicines and healthy foods. Phosphodiesterase-5 inhibitors were used as experimental examples. Through the analysis of the structure and mass spectrum characteristics of the com-pounds,phosphodiesterase-5 inhibitors were classified,and their common product ions were screened by full scan of product ions of typical compounds. Then high performance liquid chro-matography-tandem mass spectrometry( HPLC-MS / MS)method with precursor ion scanning mode was established based on the optimization of MS parameters. The effect of mass parame-ters and the choice of fragment ions were also studied. The method was applied to determine actual samples and further refined. The results demonstrated that this method can meet the need of rapid screening of unknown derivatives of phosphodiesterase-5 inhibitors in complex matrix,and prevent unknown derivatives undetected. This method shows advantages in sensi-tivity,specificity and efficiency,and is worth to be further investigated.关键词
液相色谱-串联质谱/前体离子扫描/母离子扫描/5 型磷酸二酯酶抑制剂/中成药/保健食品Key words
liquid chromatography-tandem mass spectrometry( LC-MS/MS)/precursor ion scanning/parent ion scanning/phosphodiesterase-5 inhibitors/Chinese traditional patent medi-cines/healthy foods分类
化学化工引用本文复制引用
孙晶,曹玲,冯有龙,谭力..前体离子扫描法在快速筛查中药及保健食品中非法添加的5型磷酸二酯酶抑制剂及其未知衍生物中的应用[J].色谱,2014,(11):1187-1196,10.基金项目
国家科技支撑计划项目（2012BAK08B02）． （）
