色谱Issue(11)：1197-1200,4.DOI:10.3724/SP.J.1123.2014.07002
液相色谱-串联质谱法检测人血浆及脂蛋白唾液酸的含量
Measurement of sialic acid from lipoproteins and human plasma by liquid chromatography-tandem mass spectrometry
摘要
Abstract
A liquid chromatography-tandem mass spectrometry ( LC-MS / MS ) method was established to quantify sialic acid( N-acetylneuraminic acid,NANA ) from lipoproteins and human plasma. The method was used to investigate the different contents of NANA from lipo-proteins between diabetic with an average age of 51. 6 years and healthy participants with an average age of 50. 7 years. The NANA from lipoprotein samples was hydrolyzed by acetic acid (pH = 2)at 80 ℃ for 2 h and analyzed by the optimized LC-MS / MS method after high speed centrifugation and filtration. The limits of detection and quantification of NANA were 7. 4 and 24. 5 pg,respectively. The linear range was 2. 5-80 ng / mL for NANA and the correlation coeffi-cient(R2 )was more than 0. 998. The levels of NANA in the plasma of type Ⅱ diabetics and healthy participants were(548. 3±88. 9)and(415. 3±55. 5)mg / L,respectively;and the levels of NANA from very low density lipoproteins(VLDL),low density lipoproteins(LDL),and high density lipoproteins(HDL)of the type Ⅱ diabetics and the healthy participants were( 4. 91 ± 0. 19),(6. 95± 0. 28),(3. 61 ± 0. 22)μg / mg and( 2. 90 ± 0. 27),( 7. 03 ± 0. 04),( 2. 40 ± 0. 09)μg / mg,respectively. The sialic acid levels of VLDL and HDL from the type Ⅱ diabetics were markedly higher than those of the corresponding healthy participants with the similar ages(P<0. 01). The method can rapidly determine the sialic acid from lipoproteins,and is reproducible and time saving.关键词
液相色谱-串联质谱/唾液酸/脂蛋白/人血浆/糖尿病Key words
liquid chromatography-tandem mass spectrometry(LC-MS/MS)/sialic acid/lipo-protein/human plasma/diabetes mellitus分类
化学化工引用本文复制引用
郭守东,桑慧,杨娜娜,阚玉杰,李富裕,李煜,李方圆,秦树存..液相色谱-串联质谱法检测人血浆及脂蛋白唾液酸的含量[J].色谱,2014,(11):1197-1200,4.基金项目
国家自然科学基金（31300639，81170785）；山东省青年基金项目（ ZR2013HQ014）；山东省中医药科学技术研究项目（2013-257）；泰山医学院博士启动基金项目（2234）． （）
