色谱Issue(11)：1225-1229,5.DOI:10.3724/SP.J.1123.2014.06040
1-乙基-3-甲基咪唑 L-乳酸盐作为手性配体用于氨基酸的手性拆分
Application of 1-ethyl-3-methyl imidazole L-lactate as the chiral ligand for the enantioseparation of amino acids
摘要
Abstract
Enantioseparation of tryptophan enantiomers( Trys),tyrosine enantiomers( Tyrs) and phenylamine enantiomers(Phes)by chiral ligand exchange capillary electrophoresis(CLE-CE)using 1-ethyl-3-methyl imidazole L-lactate([ EMIM][ L-Lac]) as the chiral ligand was studied. The effects of background electrolytes and central ions,the molar ratio and concentra-tion of the chiral ligand and central ion,running buffer solution pH on the enantioseparation of Trys,Phes and Tyrs were investigated. Good enantioseparation of three pairs of enantiomers were obtained with 40. 0 mmol / L[EMIM][L-Lac]and 20. 0 mmol / L cupric chloride(pH 4. 5). To validate the good behavior of[ EMIM][ L-Lac],L-lactate( L-Lac)was further used as the chiral ligand for the enantioseparation of Trys. The contrast experiment showed that Trys could only be partially enantioseparated by L-Lac. Moreover,the enantioseparation was improved by adding 1-ethyl-3-methyl imidazole acetate( EMIM-Ace)which markedly prolonged the migra-tion time of Trys to more than 30 min. In contrast,the migration time of Trys was obtained in 15 min by[ EMIM][ L-Lac]. Finally,the enantioseparation mechanism was further discussed. The experimental results showed that in the EMIM-Ace assisting L-Lac system,EMIM-Ace was only used to suppress the electroosmotic flow and was not involved in the chiral ligand exchange reaction,while in the[EMIM][L-Lac]system,the chiral ligand taking part in the re-action was mainly unionized[EMIM][L-Lac]rather than L-Lac formed by ionized L-Lac ions.关键词
手性离子液体/1-乙基-3-甲基咪唑 L-乳酸盐/手性配体交换毛细管电泳/手性分离Key words
chiral ionic liquid/1-ethyl-3-methyl imidazole L-lactate/chiral ligand exchange capillary electrophoresis/chiral separation分类
化学化工引用本文复制引用
黄露,余丽双,陈毅挺..1-乙基-3-甲基咪唑 L-乳酸盐作为手性配体用于氨基酸的手性拆分[J].色谱,2014,(11):1225-1229,5.基金项目
国家自然科学基金项目（81202913）；福建省中青年教师教育科研项目（ JA14248）． （）
