色谱Issue(12)：1368-1372,5.DOI:10.3724/SP.J.1123.2014.09008
基于介孔碳的电化学酪氨酸酶生物传感器法测定水体中的苯酚及高效液相色谱法评价
Determination of phenol in water by electrochemical tyrosinase biosensor based on ordered graphitized mesoporous carbon and evaluated by high performance liquid chromatography
摘要
Abstract
A novel electrochemical tyrosinase biosensor based on ordered graphitized meso-porous carbon( GMC)was obtained,which was used as a platform for phenol detection. The accuracy of tyrosinase biosensor method was comparatively evaluated by high performance liquid chromatography(HPLC). By entrapping tyrosinase molecules(6. 5 nm×9. 8 nm×5. 5 nm) into the mesopores of GMC( diameter 10 nm,GMC10),the“interspace confinement effect”of GMC10 may improve the stability of tyrosinase in vitro. After 21-day storage,the GMC10-based tyrosinase biosensor retained more than 85% of its initial response. It is indicated that GMC10 with“interspace confinement effect”can significantly prolong the life of tyrosinase molecules in vitro. Furthermore, the GMC-based tyrosinase biosensor displayed excellent analytical performances for phenol detection,such as stability,repeatability,selectivity,sensitivity and limit of detection. The GMC-based tyrosinase biosensor demonstrated a linear response for phenol from 0. 1 to 10 μmol/L with a low detection limit of 20 nmol/L. The comparative study between HPLC and GMC-based tyrosinase biosensor showed that the detection of phenol in water sample by the GMC-based tyrosinase biosensor method is reliable,accurate and effective. The proposed GMC-based tyrosinase biosensor proved to be a very promising“pre-alarm”tool for rapid detecting phenol pollution in emergency accidents.关键词
高效液相色谱/电化学生物传感器/介孔碳/酪氨酸酶/苯酚/水体Key words
high performance liquid chromatography ( HPLC )/electrochemical biosensor/graphitized mesoporous carbon( GMC)/tyrosinase/phenol/water分类
化学化工引用本文复制引用
吴立冬,刘欢,李晋成,付啸辰,宋怿..基于介孔碳的电化学酪氨酸酶生物传感器法测定水体中的苯酚及高效液相色谱法评价[J].色谱,2014,(12):1368-1372,5.基金项目
国家自然科学基金项目（21307161）；中央级公益性科研院所基本科研业务费专项资金项目（2013C006）． （）
