色谱Issue(12)：1400-1403,4.DOI:10.3724/SP.J.1123.2014.07037
高效阴离子交换色谱-脉冲安培检测法同时测定生物转化样品中的海藻糖、葡萄糖和麦芽糖
Simultaneous analysis of trehalose,glucose and maltose in biotransformation samples by high performance anion exchange chromatography with pulsed ampere detection
摘要
Abstract
An analytical method for the determination of trehalose,maltose,and glucose in biotransformation samples was developed by using high performance anion exchange chroma-tography coupled with pulsed ampere detection( HPAEC-PAD). The analysis was performed on a CarboPacTM 10 column( 250 mm × 2 mm ) with the gradient elution of NaOH-NaAc as the mobile phase. The column temperature was set at 30 ℃,the flow rate was 0. 30 mL/min. The results showed that trehalose,maltose,and glucose in biotransformation system were com-pletely separated and determined in 15 min. The linear ranges and the working curves were determined by using standard samples. The correlation coefficients of three kinds of carbohy-drates were over 0. 999 8 . The detection limits( LODs)were 0. 010-0. 100 mg/L. Under the optimized separation conditions,the recoveries of saccharides in the transformation system at three different spiked levels ranged from 89. 4% to 103. 2%. In biotransformation system,50 IU trehalose synthase were added into 200 g/L maltose for reaction of 8 h at 37 ℃,pH 8. 0. Under the above conditions,the concentration of trehalose in biotransformation sample was 101. 084 g/L,and the conversion rate of trehalose reached 50. 5%. The method can be applied to deter-mine the composition in the transformation system with the advantages of simplicity and con-venience.关键词
高效阴离子交换色谱/海藻糖/葡萄糖/麦芽糖/生物转化样品Key words
high performance anion exchange chromatography( HPAEC)/trehalose/glucose/maltose/biotransformation sample分类
化学化工引用本文复制引用
徐颖,臧颖,姜婷,郑兆娟,欧阳嘉..高效阴离子交换色谱-脉冲安培检测法同时测定生物转化样品中的海藻糖、葡萄糖和麦芽糖[J].色谱,2014,(12):1400-1403,4.基金项目
国家自然科学基金项目（31200443）；江苏省杰出青年基金项目（BK2012038）；新世纪优秀人才支持计划项目（NCET-11-0988）；江苏高校优势学科建设工程项目． （）
