色谱Issue(1)：75-79,5.DOI:10.3724/SP.J.1123.2014.10012
高效液相色谱法测定水基胶黏剂中3种异噻唑啉酮类杀菌剂
Determination of three isothiazolinone biocides in water-borne adhesives by high performance liquid chromatography
周晓 1李小兰 1陈志燕 1叶长文 2周芸 1孟冬玲1
作者信息
- 1. 广西中烟工业有限责任公司，广西 南宁 530001
- 2. 中国烟草总公司郑州烟草研究院，河南 郑州 450001
- 折叠
摘要
Abstract
A rapid high performance liquid chromatography( HPLC)method was developed for the quantitative analysis of three isothiazolinone biocides( 2-methyl-4-isothiazolin-3-one( MI), 5-chloro-2-methyl-4-isothiazolin-3-one ( CMI ) and 1,2-benzylisothiazolin-3-one ( BIT )) in water-borne adhesives. The sample was extracted with methanol-water(1:1,v/v),and puri-fied by centrifugation and filtration. The isothiazolones were separated on a C18 column with methanol-water as mobile phases under gradient elution and detected with a diode array detector( DAD). The pretreatment factors such as extraction solvent,extraction method,dilu-tion ratio,extraction time were optimized. Under the optimized conditions,the targets had good linearities( r2≥0. 999 2)in the range of 0. 25-10. 0 mg/L. The recoveries were between 92% and 103% with the relative standard deviations not more than 4%. The limits of detection ( LODs)were between 0. 43 mg/kg and 1. 14 mg/kg. The limits of quantification( LOQs)were between 1. 44 mg/kg and 3. 81 mg/kg. The results showed that the method can achieve the pur-pose of quantitative detection. The analyses of real samples verified the reliability of this method.关键词
高效液相色谱/异噻唑啉酮类杀菌剂/水基胶黏剂Key words
high performance liquid chromatography( HPLC)/isothiazolone biocides/water-borne adhesives分类
化学化工引用本文复制引用
周晓,李小兰,陈志燕,叶长文,周芸,孟冬玲..高效液相色谱法测定水基胶黏剂中3种异噻唑啉酮类杀菌剂[J].色谱,2015,(1):75-79,5.
