色谱Issue(1)：80-83,4.DOI:10.3724/SP.J.1123.2014.08024
柱前衍生-高效液相色谱-荧光检测法测定罐头食品中的尿素
Determination of urea in canned foods by high performance liquid chromatography-fluorescence detection coupled with precolumn derivatization
摘要
Abstract
A method for the determination of urea residue in canned foods by high performance liquid chromatography-fluorescence detection( HPLC-FLD)coupled with precolumn derivatiza-tion was established. The sample(5. 0 g),including canned edible fungi,fruit,vegetable,fish, and meat was extracted with 20 mL 1%( v/v)acetic acid solution. The extract was centrifuged, filtrated,and then derivatized with xanthydrol. The analysis was completed with HPLC-FLD. A good linear relationship was obtained in the range of 0. 1-500 mg/L with the correlation coeffi-cients more than 0. 999 5. The average recoveries of urea spiked at 0. 001-30 g/kg levels in five kinds of canned foods ranged from 80. 2% to 109. 7% with the RSDs of 2. 05%-6. 53%. The limit of detection(LOD)was 0. 5 mg/kg,and the limit of quantitation(LOQ)was 1. 0 mg/kg. The proposed procedure was then applied to the analysis of 168 real samples collected from Xia-men,Fujian Province,China. The existence of urea was found in three pork cans with contents of 10. 6,62. 1 and 2. 6 mg/kg,respectively. The method is stable,reliable,simple and suitable for the determination of urea in canned foods,and has great potential for routine analysis in foodstuffs.关键词
高效液相色谱/荧光检测/尿素/罐头食品/呫吨醇Key words
high performance liquid chromatography( HPLC)/fluorescence detection( FLD)/urea/canned food/xanthydrol分类
化学化工引用本文复制引用
曾琪,张缙,徐敦明,张志刚,柯志成..柱前衍生-高效液相色谱-荧光检测法测定罐头食品中的尿素[J].色谱,2015,(1):80-83,4.基金项目
国家质检总局科技项目（2012IK165）；国家认监委制标项目（2009B739r）． （）
