色谱Issue(3)：221-227,7.DOI:10.3724/SP.J.1123.2014.11022
新型核壳型亲水聚合物-硅胶杂化填料的合成及其在蛋白质N-糖链富集中的应用
Synthesis of core-shell hydrophilic polymer-silica hybrid material and its application in N-glycan enrichment
摘要
Abstract
Protein N-glycosylation is one of the most important post-translational modifications closely correlated with many important biological and pathological processes. The structural alterations of N-linked glycans in glycoproteins are always associated with many diseases,such as diabetes,heart failure and malignant tumors. Therefore,it is very important to establish sen-sitive methods for high-throughput N-glycan profiling. However,the low abundance of the N-glycoproteins and the heterogeneity of the N-glycans make it a challenge to analyse the pro-tein glycosylation sensitively. In this work,we had synthesized core-shell hydrophilic polymer-silica hybrid materials(pGMAG-SiO2)for the efficient enrichment of protein N-glycans. Firstly, pGMAG-SiO2 was prepared by in situ growth of glucose polymer on the surface of silica microp-articles using surface-initiated atom transfer radical polymerization( SI-ATRP)technique. The strong hydrophilicity of the material makes it suitable for the enrichment of N-glycans released from complex samples. Secondly,maltoheptaose and the N-glycans from chicken egg albumin were used as standard samples to optimize the enrichment conditions and evaluate the enrich-ment efficiency of pGMAG-SiO2. Finally,pGMAG-SiO2 was applied to the enrichment of N-linked glycans from human plasma proteins and 47 glycoforms were successfully identified after enrichment. These results demonstrated the high enrichment efficiency and significant applica-tion value of pGMAG-SiO2 in the analysis of N-glycans.关键词
表面引发-原子转移自由基聚合反应/核壳型硅胶填料/N-糖链/N-糖基化蛋白质Key words
surface-initiated atom transfer radical polymerization( SI-ATRP)/core-shell silica packing/N-glycan/N-glycoprotein分类
化学化工引用本文复制引用
白海红,范超,沈丙权,田芳,邓玉林,潘一廷,秦伟捷,钱小红..新型核壳型亲水聚合物-硅胶杂化填料的合成及其在蛋白质N-糖链富集中的应用[J].色谱,2015,(3):221-227,7.基金项目
国家重大科学计划项目（2013CB911204）；国家重大科学仪器设备开发专项项目（2011YQ09000504）；国家自然科学基金项目（21275005，21235001）． （）
