色谱Issue(3)：275-281,7.DOI:10.3724/SP.J.1123.2014.10029
液相色谱-串联质谱法同时测定化妆品中的25种喹诺酮类药物
Determination of 25 quinolones in cosmetics by liquid chromatography-tandem mass spectrometry
摘要
Abstract
An analytical method was developed for the simultaneous determination of 25 quino-lones,including danofloxacin mesylate,enrofloxacin,flumequine,oxloinic acid,ciprofloxa-cin,sarafloxacin, nalidixic acid, norfloxacin, and ofloxacin etc in cosmetics using direct extraction and liquid chromatography-electrospray ionization tandem mass spectrometry( LC-ESI-MS/MS). Cosmetic sample was extracted by acidified acetonitrile,defatted by n-hexane and separated on Poroshell EC-C 18 column with gradient elution program using acetonitrile and water(both containing 0. 1% formic acid)as the mobile phases and analyzed by LC-ESI-MS/MS under the positive mode using multiple reaction monitoring( MRM). The interference of ma-trix was reduced by the matrix-matched calibration standard curve. The method showed good linearities over the range of 1—200 mg/kg for the 25 quinolones with good linear correlation coefficients( r≥0. 999). The method detection limit of the 25 quinolones was 1. 0 mg/kg,and the recoveries of all analytes in lotion,milky and cream cosmetics matrices ranged from 87. 4%to 105% at the spiked levels of 1,5 and 10 mg/kg with the relative standard deviations( RSD) of 4. 54%—19. 7%( n=6). The results indicated that this method is simple,fast and credible, and suitable for the simultaneous determination of the quinolones in the above three types of cosmetics.关键词
液相色谱-电喷雾电离串联质谱/喹诺酮/化妆品Key words
liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESI-MS/MS)/quinolones/cosmetics分类
化学化工引用本文复制引用
林黎,张毅,涂小珂,谢丽琪,岳振峰,康海宁,吴卫东,罗耀..液相色谱-串联质谱法同时测定化妆品中的25种喹诺酮类药物[J].色谱,2015,(3):275-281,7.基金项目
国家自然科学基金项目（21107074）；深圳市基础研究项目（JCYJ2012061872144497，JCY201110097）． （）
