色谱Issue(3):318-322,5.DOI:10.3724/SP.J.1123.2014.11005
应用动态顶空吸附-气相色谱-质谱法分析算盘子花气味的化学成分
Analysis of the components of floral scent in Glochidion puberum using gas chromatography-mass spectrometry with dynamic headspace adsorption
摘要
Abstract
The floral scent plays the important key role in maintaining the obligate pollination mutualism between Glochidion plants and Epicephala moths. In the study,the dynamic head-space adsorption technique was employed to collect the floral scent emitted by Glochidion pu-berum,gas chromatography coupled with mass spectrometry( GC-MS)was used for the detec-tion and identification of volatile chemical components in headspace samples of flowers from G. puberum. The peak area normalization was used to determine the relative contents of each odour component. The results showed that 45 compounds mainly consisting of monoterpenes and sesquiterpenes were isolated from the floral scent produced by G. puberum. Especially, both linalool( 38. 06%)and β-elemene( 23. 84%)were considered as the major scent compo-nents of G. puberum. It was speculated that linalool and β-elemene may be the two potential compounds attracting female Epicephala moths. The study provided the basic data for further electroantennographic detection and bioassays to identify the compounds having the actual physiological activity to female Epicephala moths.关键词
动态顶空吸附/气相色谱-质谱/花气味/算盘子/互利共生关系Key words
dynamic headspace adsorption(DHSA)/gas chromatography-mass spectrometry (GC-MS)/floral scent/Glochidion puberum/mutualism分类
化学化工引用本文复制引用
黄代红,张振国,陈国平,李后魂,石福臣..应用动态顶空吸附-气相色谱-质谱法分析算盘子花气味的化学成分[J].色谱,2015,(3):318-322,5.基金项目
国家自然科学基金重点项目(30930014). ()