色谱Issue(5)：475-480,6.DOI:10.3724/SP.J.1123.2015.01008
牛血清白蛋白和溶菌酶为双模板蛋白质的表面印迹聚合物的制备及吸附性能
Preparation and adsorption characteristics of surface-imprinted polymer using bovine serum albumin and lysozyme as double template proteins
盖青青 1屈锋2
作者信息
- 1. 北京低碳清洁能源研究所，北京 102209
- 2. 北京理工大学生命学院，北京 100081
- 折叠
摘要
Abstract
The double template surface-imprinted polymer( Bi-MIP )was synthesized by atom transfer radical polymerization( ATRP)in aqueous media,using bovine serum albumin( BSA) and lysozyme( Lyz)as the template proteins,N-isopropylacrylamide( NIPAAm)and acrylam-ide(AAm)as the monomers and N-[3-(dimethylamino)propyl]-methacrylamide(DMAPMA) as the assistant of basic functional monomer. The ATRP possessed the mild reaction conditions and can be initiated at room temperature without heating and ultraviolet radiations. The prepa-ration conditions of imprinted polymer were optimized,and the content of DMAPMA as the assistant of basic functional monomer was investigated. The results showed that Bi-MIP exhibi-ted the good adsorption capacity and selectivity in single protein solution and in mixed protein solution when the volume of DMAPMA in the preparation process of Bi-MIP was 20 μL. The rebinding capacity of Bi-MIP was evaluated according to adsorption isotherm of the imprinted polymer. The Langmuir adsorption model was employed to describe the isotherms and revealed that Bi-MIP exhibited the maximum rebinding to BSA and Lyz at 10. 2 mg/g and 19. 2 mg/g, respectively. And Bi-MIP had good imprinting effect and adsorption capacity to templates BSA and Lyz from egg white and bovine serum samples. This will provide a new way for specific rec-ognition of the double/multiple target proteins in the complex biological samples.关键词
表面分子印迹聚合物/双模板蛋白质/吸附性能Key words
surface molecularly imprinted polymer/double template proteins/adsorption char-acteristics分类
化学化工引用本文复制引用
盖青青,屈锋..牛血清白蛋白和溶菌酶为双模板蛋白质的表面印迹聚合物的制备及吸附性能[J].色谱,2015,(5):475-480,6.
