色谱Issue(5)：530-534,5.DOI:10.3724/SP.J.1123.2015.01003
细胞膜色谱法研究5-羟色胺受体与藁本内酯的亲和作用
A cell membrane chromatography method for investigation of 5-hydroxytryptamine receptor-ligustilide interaction
摘要
Abstract
A rat striatum cell membrane chromatography( CMC)frontal analysis method was developed for the determination of the equilibrium dissociation constants ( KD) for 5-hydroxytryptamine(5-HT)receptor 5-HT1D-ligustilide interactions. Rat striatum was used for preparation of the cell membrane stationary phase( CMSP). An enzyme-linked immunosorbent assay( ELISA)was applied to determine the 5-HT level of CMSP before and after the adsorp-tion of cell membrane,and the value was(40. 5±2. 3)pg per gram of silica. The CMC-offline-HPLC system was applied to specifically recognize the mixed standard solution of sumatriptan and ligustilide. Sumatriptan,a 5-HT1D agonist of 24. 2 to 242 nmol/L,was pumped through a CMC column continuously,and the breakthrough curves were recorded. For further competitive studies,the mobile phase that contained ligustilide(37. 0-370 nmol/L)was pumped through the column to saturate the binding sites. Afterwards,sumatriptan was propelled towards the column. The breakthrough curves were recorded and compared with those obtained from the column without saturation. KD values obtained using frontal analysis were 389 nmol/L and 4. 21μmol/L for sumatriptan and ligustilide,respectively. The competitive binding study indicated that the CMC method could be a quick and efficient way for determining the KDvalues in drug-receptor interactions.关键词
细胞膜色谱/前沿分析/舒马普坦/藁本内酯/5-羟色胺受体Key words
cell membrane chromatography( CMC)/frontal analysis/sumatriptan/ligustilide/5-hydroxytryptamine(5-HT)receptor分类
化学化工引用本文复制引用
杜晖,周娜,李静静,范菲..细胞膜色谱法研究5-羟色胺受体与藁本内酯的亲和作用[J].色谱,2015,(5):530-534,5.基金项目
陕西省中医药管理局科研课题（13-ZY005）． （）
