色谱Issue(5)：541-544,4.DOI:10.3724/SP.J.1123.2015.01023
高效液相色谱-串联质谱法测定卷烟中的儿茶酚
Determination of catechol in tobacco by high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
An analytical method for the determination of catechol in tobacco by high perform-ance liquid chromatography-tandem mass spectrometry( HPLC-MS/MS)has been developed. A Sep-Park-C18 solid phase extraction cartridge was used for the enrichment of the analyte for HPLC-MS/MS analysis. The mobile phase was methanol-0. 2%( v/v)formic acid with gradient elution. The sample was analyzed by HPLC-MS/MS in the ESI-scanning mode with multi-reac-tion monitoring( MRM)for qualitative and quantitative analyses. The linear range of calibration curve was 0. 5-200 μg/kg with good correlation coefficients( r 2=0. 998 9 ). The recoveries of catechol spiked in three levels were in the range of 83. 1%-98. 6%,with the relative standard deviations of 1. 9%-5. 8%. This method was initially applied to the research of catechol as a fla-vor additive in six retail tobacco samples and proved to be accurate,sensitive,convenient and practical. The analyte was found in the six retail tobacco samples,and in some cases,the pres-ence of quite high concentrations of catechol in tobacco should be a matter of concern.关键词
固相萃取/高效液相色谱-串联质谱法/儿茶酚/卷烟Key words
solid phase extraction(SPE)/high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)/catechol/tobacco分类
化学化工引用本文复制引用
任志芹,艾小勇,黄志强,张元,王志,张峰..高效液相色谱-串联质谱法测定卷烟中的儿茶酚[J].色谱,2015,(5):541-544,4.基金项目
质检公益性行业科研专项（201410088，201310134）；国家质检总局科技计划项目（2013IK202）；国家重大仪器设备开发专项（2012YQ14000806）． （）
