色谱Issue(6)：558-562,5.DOI:10.3724/SP.J.1123.2015.01013
梯度液相色谱中梯度曲线变形对色谱峰宽的影响
Influence of the distortion of gradient profile on peak width in gradient liquid chromatography
摘要
Abstract
In gradient liquid chromatography,the gradient profile may be distorted because of the effects of solvent mixing and axial dispersion. Such effects will be significant especially when stepwise gradient and linear gradient with high slope are applied. In this work,we dis-cussed the influence of the distortion of gradient profile on the peak width. Firstly,the chroma-tographic peaks were measured under linear and stepwise gradient conditions where a C18 col-umn was used as the stationary phase,a mixture of methanol and water as the mobile phase, and biphenyl and acetophenone as the samples. Then,the response of methanol under corre-sponding conditions was recorded by disconnecting the column and the detection wavelength was set at 205 nm. The gradient profile at the column inlet was calculated from the methanol response profile. The theoretical values of the peak width were calculated from the set gradient profile and the gradient profile obtained at the column inlet respectively. The theoretical values were compared with the experimental ones. It is shown that the distortion of gradient profile does affect the peak width. When such influence is taken into account,the theoretical and experimental values of the peak width are well consistent.关键词
液相色谱/梯度洗脱/梯度曲线/色谱峰宽Key words
liquid chromatography/gradient elution/gradient profile/peak width分类
化学化工引用本文复制引用
吴顺,郝卫强,岳邦毅,张培培,狄斌,陈强..梯度液相色谱中梯度曲线变形对色谱峰宽的影响[J].色谱,2015,(6):558-562,5.基金项目
国家自然科学基金项目（21005009，81173022）；江苏省自然科学基金项目（BK20141163）；烟草行业卷烟烟气重点实验室开放研究基金项目（ SZBCW201300555）；常州市应用基础研究计划项目（ CJ20115002）． （）
