色谱Issue(6)：583-589,7.DOI:10.3724/SP.J.1123.2015.01040
超高效液相色谱-线性离子阱／静电场轨道阱高分辨质谱直接分析葡萄酒中38种多酚类化合物
Direct analysis of 38 polyphenols in wine by ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry
摘要
Abstract
Wine has been described previously as a rich source of polyphenols. However,an accurate screening of its complete phenolic profile is still lacking. In the present work,the anal-ysis of 38 polyphenols in wine using ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry ( UPLC-LTQ/Orbitrap MS ) was explored. Wines were directly detected. The sample was loaded onto a Thermo Hypersil Gold C18 column (100 mm × 2. 1 mm,1. 9 μm)using a gradient elution of acetonitrile/water containing 0. 1%( v/v)formic acid for the separation. UPLC-LTQ/Orbitrap mass spectrometer acquired full scan MS date for quantification,and data dependent MS2 product ion spectra for identification and/or confirmation. The regression coefficients(R2)for the calibration curves(two orders of mag-nitude up to the lowest calibration level)in the study were ≥0. 99. The limits of detection for the 38 compounds were 0. 002-0. 50 mg/kg. The average recoveries at three spiked levels were in the range of 90%-102% with the relative standard deviations( RSDs)of 0. 51%-2. 56%. Mass errors were always ≤ 5 ppm. This procedure was then successfully applied to the analysis of the polyphenols in wines.关键词
超高效液相色谱/线性离子阱/静电场轨道阱质谱/高分辨质谱/多酚化合物/葡萄酒Key words
ultra high performance liquid chromatography(UPLC )/linear ion trap/orbitrap mass spectrometry(LTQ/Orbitrap MS)/high resolution mass spectrometry(HRMS)/polyphe-nols/wine分类
化学化工引用本文复制引用
张协光,郑彦婕,曾泳艇,刘文丽..超高效液相色谱-线性离子阱／静电场轨道阱高分辨质谱直接分析葡萄酒中38种多酚类化合物[J].色谱,2015,(6):583-589,7.基金项目
深圳市技术攻关项目（ JSGG20140519114050315）． （）
