色谱Issue(6)：613-621,9.DOI:10.3724/SP.J.1123.2015.01028
基于柱前衍生-超高效液相色谱-质谱联用技术的植物提取液中氨基化合物代谢谱分析
Metabolic profiling analysis of amino metabolites in plant extract based on pre-column derivatization-ultra high performance liquid chromatography-mass spectrometry
张俊杰 1赵春霞 1赵燕妮 1赵洁妤 1李丽丽 1路鑫 1许国旺1
作者信息
- 1. 中国科学院大连化学物理研究所，中国科学院分离分析化学重点实验室，辽宁 大连 116023
- 折叠
摘要
Abstract
Amino metabolites are important compounds that play a key role in plant growth and development. A metabolic profiling analysis method of amino metabolites in plant extract was developed based on pre-column derivatization-ultra high performance liquid chromatography-mass spectrometry. Using the tobacco leaf as an example,a total of 87 amino metabolites, including amino acids,amines,peptides,alkaloids etc. were detected. The repeatability of the method was good with RSDs of 85 amino metabolites between 1. 5% and 18. 8%. Forty-three amino metabolites validated by standard samples showed good linearity with the correlation coefficients of 0. 993-0. 999,covered linear range of four orders of magnitude. The limits of detection were 0. 03-6. 58 ng/mL. The intra-day and inter-day precisions were 0. 7%-15. 6% and 0. 8%-22. 9%,respectively. The recoveries were 74. 4%-122. 7%. The influence of topping on metabolic profiling of amino metabolites in fresh tobacco was investigated using the developed method. The results showed that the amino metabolites in the upper tobacco leaves were most affected than those in the middle and lower leaves. Metabolism of amino metabolites in the upper leaves after topping was mainly towards the alkaloid synthesis. The method integrated the advantages of triple quadrupole mass spectrometry and high resolution quadrupole-time of flight mass spectrometry. It can be used for metabolic profiling analysis of amino metabolites in plant extract with high sensitivity and selectivity.关键词
柱前衍生/超高效液相色谱/四极杆飞行时间质谱/三重四极杆质谱/氨基化合物/代谢谱/植物提取液Key words
pre-column derivatization/ultra high performance liquid chromatography(UHPLC)/quadrupole-time of flight mass spectrometry( Q-TOF MS)/triple quadrupole mass /spectrometry( QQQ MS)/amino metabolite/metabolic profiling/plant extract分类
化学化工引用本文复制引用
张俊杰,赵春霞,赵燕妮,赵洁妤,李丽丽,路鑫,许国旺..基于柱前衍生-超高效液相色谱-质谱联用技术的植物提取液中氨基化合物代谢谱分析[J].色谱,2015,(6):613-621,9.
