色谱Issue(6)：657-661,5.DOI:10.3724/SP.J.1123.2015.01046
改进的QuEChERS方法结合液相色谱-串联质谱法测定腐竹和豆干中的二甲基黄和二乙基黄
Determination of dimethyl yellow and diethyl yellow in yuba and dried beancurd by modified QuEChERS method and liquid chromatography-tandem mass spectrometry
范素芳 1李强 1马俊美 1李挥 1张岩1
作者信息
- 1. 河北省食品检验研究院，河北省食品安全重点实验室，河北 石家庄 050091
- 折叠
摘要
Abstract
A modified QuEChERS( quick,easy,cheap,effective,rugged and safe)method followed by liquid chromatography-tandem mass spectrometric analysis was developed for the determination of dimethyl yellow and diethyl yellow in yuba and dried beancurd. Yuba and dried beancurd samples were soaked by deionized water,then acetonitrile was added to extract the analytes. Sodium chloride and anhydrous magnesium sulfate were added for liquid-liquid separa-tion. The extracts were cleaned-up by dispersive solid-phase using N-propyl diethylamine. The analytes were separated by liquid chromatography and determined by mass spectrometry. Exter-nal standard method was used for quantification. The recoveries of dimethyl yellow were in the range of 73. 5%-84. 5% at spiked levels of 0. 3,1 and 10 μg/kg and the recoveries of diethyl yellow were in range of 70. 5%-81. 2% at spiked levels of 0. 1,1 and 10 μg/kg;relative stand-ard deviations of the method were lower than 11%. The limit of detection and the limit of quan-tification of dimethyl yellow were 0. 1 μg/kg and 0. 3 μg/kg,respectively;the limit of detection and the limit of quantification of diethyl yellow were 0. 05 μg/kg and 0. 1 μg/kg,respectively. This method can be used in rapid screening and quantitative analysis of dimethyl yellow and diethyl yellow in yuba and dried beancurd.关键词
QuEChERS/液相色谱-串联质谱/二甲基黄/二乙基黄/腐竹/豆干Key words
QuEChERS/liquid chromatography-tandem mass spectrometry(LC-MS/MS )/dimethyl yellow/diethyl yellow/yuba/dried beancurd分类
化学化工引用本文复制引用
范素芳,李强,马俊美,李挥,张岩..改进的QuEChERS方法结合液相色谱-串联质谱法测定腐竹和豆干中的二甲基黄和二乙基黄[J].色谱,2015,(6):657-661,5.
