色谱Issue(7)：673-677,5.DOI:10.3724/SP.J.1123.2015.03026
软骨藻酸作用蛋白质的亲和毛细管电泳筛选
Affinity capillary electrophoresis for screening proteins interacting with domoic acid
摘要
Abstract
Domoic acid( DA)is a biological neurotoxin that causes amnesic shellfish poison-ing. Study of the interactions between DA and important functional proteins contributes to understand the toxicity mechanism of DA to biological macromolecules. In this paper,the inter-actions between DA and nine important proteins in plasma,intestine and mitochondria were qualitatively compared by affinity capillary electrophoresis. Proteins were used as affinity lig-ands while DA as the affinity receptor. Proteins with the concentrations of 0,0. 2,0. 4,0. 6, 0. 8 μmol/L were added in the electrophoresis buffer and the migration times of 0. 2 mg/mL DA were detected. Then the linear graphs of the variation of DA mobility ratio(ΔM)with the pro-tein mass concentration( L)were drawn. According to the slope value,the relative strength of the interactions between DA and proteins was compared. The results showed that six proteins can interact with DA and the relative strength order was human thrombin>cytochrome C≈tryp-sin≈immunoglobulin E( Ig E)≈ribonuclease A>λ exonuclease,while ferritin,transferrin and lectin had no affinity with DA. With the advantages of high efficiency,fast analysis and less sample consumption,affinity capillary electrophoresis is a convenient method for screening DA target proteins,which will provide basic information for the toxic mechanism and defence of DA.关键词
亲和毛细管电泳/软骨藻酸/蛋白质/相互作用Key words
affinity capillary electrophoresis/domoic acid/protein/interaction分类
化学化工引用本文复制引用
王晓倩,高铁,洪专,屈锋..软骨藻酸作用蛋白质的亲和毛细管电泳筛选[J].色谱,2015,(7):673-677,5.基金项目
国家自然科学基金项目（21175011，21375008）；国家“973”计划项目（2012CB910603）；国家海洋局海洋生物资源综合利用工程技术研究中心开放基金（ MBRCU201304）． （）
