色谱Issue(7)：711-714,4.DOI:10.3724/SP.J.1123.2015.02057
超高效液相色谱-高分辨质谱法测定蜂蜜中的3种马桑内酯
Determination of three coriaria lactones in honey by ultra high performance liquid chromatography-high resolution mass spectrometry
摘要
Abstract
A method for the determination of three coriaria lactone residues in honey was developed using ultra high performance liquid chromatography-high resolution mass spectrome-try. The honey samples were extracted with 0. 2 mol/L phosphate buffer solution( pH=7. 5), and the extracts were cleaned up with Waters HLB solid phase extraction cartridges. The extracted components were separated on a Phenomenex C18 column by gradient elution. The qualitative and quantitative analyses were operated under t-MS 2 by high resolution mass spec-trometry. The results showed that the limits of detection and quantification for the three coriaria lactones in a spiked blank honey were 0. 05 mg/kg and 0. 1 mg/kg,respectively. The recoveries of the three coriaria lactones spiked in blank honey samples at the levels of 0. 1 to 0. 5 mg/kg were 86. 3%-95. 6% with the RSDs of 3. 0%-8. 4%. The method was applied for the determina-tion of the manuka honey from New Zealand,and tutin was detected in one of the samples. The results showed that the method is suitable for the determination of the three coriaria lactone residues in honey.关键词
超高效液相色谱/高分辨质谱/马桑内酯/蜂蜜Key words
ultra high performance liquid chromatography( UPLC)/high resolution mass spec-trometry( HRMS)/coriaria lactones/honey分类
化学化工引用本文复制引用
殷耀,陈恵兰,陈磊,别小妹,丁涛,张晓燕,吴斌,沈崇钰,张睿..超高效液相色谱-高分辨质谱法测定蜂蜜中的3种马桑内酯[J].色谱,2015,(7):711-714,4.基金项目
江苏省第4期“333工程”科研项目（ BRA2013276）；江苏省大型科学仪器设备共享服务平台（ BZ201302）． （）
