色谱Issue(7)：740-745,6.DOI:10.3724/SP.J.1123.2015.03005
高效液相色谱-串联质谱法同时测定水中13种有机污染物
Simultaneous determination of 13 organic pollutants in water samples by high performance liquid chromatography-tandem mass spectrometry
罗碧容 1钱蜀 1谢振伟 1姚欢 1熊杰 1赵红1
作者信息
- 1. 四川省环境监测总站，四川 成都 610091
- 折叠
摘要
Abstract
On the basis of environmental quality standards for surface water,a sensitive and simple method was developed for the simultaneous determination of 13 organic pollutants, including dimethoate,dichlorvos,trichlorfon,parathion,methyl parathion,malathion,deme-ton,acrylamide,aniline,benzidine,carbaryl,Microcystin-LR and atrazine in water samples by high performance liquid chromatography-tandem mass spectrometry( HPLC-MS/MS)combined with direct injection. The water samples were filtered through 0. 22 μm of microfiltration mem-brane. With methanol-0. 01% formic acid as mobile phase,the separation was performed on a Kromasil 100-5 C18 column(150 mm×2. 1 mm,5 μm)in gradient elution. The flow rate was 0. 5 mL/min. The column temperature was 40 ℃. The samples were detected by multiple reac-tion monitoring( MRM ) mode with positive electro spray ionization. The organic pollutants were quantified by external standard method. The calibration curves of the organic pollutants show good linearity in suitable ranges with correlation coefficients( r)over 0. 999 5. The detec-tion limits of organic pollutants in samples ranged from 0. 02 μg/L to 0. 1 μg/L. The relative standard deviations of organic pollutants were 0. 5%-5. 0%( n=6). The average recoveries of the 13 organic pollutants spiked in water samples ranged from 81. 2% to 112%. The method has been proven to be sensitive,rapid and with little interference. It is suitable for the determina-tion of the 13 organic pollutants simultaneously in the surface and underground waters.关键词
高效液相色谱-串联质谱法/有机污染物/水样/直接进样Key words
high performance liquid chromatography-tandem mass spectrometry( HPLC-MS/MS)/organic pollutants/water samples/direct injection分类
化学化工引用本文复制引用
罗碧容,钱蜀,谢振伟,姚欢,熊杰,赵红..高效液相色谱-串联质谱法同时测定水中13种有机污染物[J].色谱,2015,(7):740-745,6.
