色谱Issue(7)：746-752,7.DOI:10.3724/SP.J.1123.2015.03001
超高效液相色谱-串联质谱法定量分析烟叶中的12种类黄酮物质
Determination of 12 flavonoids in tobacco leaves using ultra-high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
Flavonoids are very important secondary metabolites for tobacco plants. They are also considered as important flavor precursors for cigarettes. A method of ultra-high perform-ance liquid chromatography-tandem mass spectrometry( UPLC-MS/MS)was established for the simultaneous determination of 12 flavonoids in tobacco leaves. The developed method deter-mined 10 more flavonoids compared to the traditional method. A solution of methanol-water-chloroform(5∶2∶2,v/v/v)was used to extract the flavonoids from tobacco leaves and remove the pigment. Instrument analysis using the UPLC-MS/MS was completed in 13 min. The method validation was performed,and the results showed that the linear correlation coefficients(r2)of all the 12 flavonoids were more than 0. 99. The limits of detection and the limits of quantifica-tion were in the range of 0. 3-100 μg/L and 1. 2-400 μg/L,respectively. Intra-day and Inter-day reproducibilities were in the range of 3. 5%-7. 4% and 5. 2%-11. 4%,respectively. The recoveries were 81. 2%-111. 9%. The established method was successfully used to analyze the flavonoids of tobacco leaves of 11 varieties. Significant concentration differences of the flavonoids were found among the determined varieties. Furthermore,significant positive corre-lation among the flavonoids with similar chemical structures( aglycones and their related glyco-sides,glycosides with the same aglycone,and similar aglycones)was found using the acquired data.关键词
超高效液相色谱-串联质谱/类黄酮/烟草Key words
ultra-high performance liquid chromatography-tandem mass spectrometry( UPLC-MS/MS)/flavonoids/tobacco分类
化学化工引用本文复制引用
李勇,林茜,逄涛,师君丽..超高效液相色谱-串联质谱法定量分析烟叶中的12种类黄酮物质[J].色谱,2015,(7):746-752,7.基金项目
中国烟草总公司资助项目（110201101003（TS-03））；中国烟草总公司云南省公司资助项目（2014YN16，2011YN02）． （）
