水生生物学报Issue(3)：459-466,8.DOI:10.7541/2014.65
添加不同益生菌对草鱼养殖水体菌群结构的影响
COMPARATIVE ANALYSIS OF MICROBIAL COMMUNITY FROM GRASS CARP CULTURE WATER SUPPLEMENTATION WITH DIFFERENT PROBIOTICS
摘要
Abstract
In this study, 454-pyrosequencing technology was employed to investigate the microbial community structure in grass carp culture water with or without different probiotics. The results showed that all three treatments remarkably increased the bacterial diversity compared to the control. In two samples (Control and CB), the dominant phylum was Proteobacteria. However, there was only moderate difference in the content of Proteobacteria and Actinobacteria be-tween sample BS and PSB. Actinobacteria and Bacteroidetes in PSB, BS, and CB were more abundant than those in the control group. The detailed analysis of Proteobacteria showed that in PSB, BS and CB samples, the majority of Proteo-bacteria consisted of αsubdivision, followed by Betaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria; in the control sample, the dominant subtype was Gammaproteobacteria, followed by Betaproteobacteria, Alphaproteobac-teria, and Deltaproteobacteria. For Bacteroidetes, Flavobacteria was the dominant subtype in treated samples but not in the control sample. At the order level we also oberseved a marked difference between the control and the treatment in terms of the composition of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria and Bacteroidetes. The results indicated that the addition of different probiotics into water could increase the diversity and alter the structure of a microbial community.关键词
益生菌/草鱼/菌群结构/454焦磷酸测序Key words
Probiotic/Grass carp/Microbial community structure/454-Pyrosequencing分类
农业科技引用本文复制引用
张小平,王一冰,邓斌,郑佳佳,胡彩虹,吴学祥,李卫芬..添加不同益生菌对草鱼养殖水体菌群结构的影响[J].水生生物学报,2014,(3):459-466,8.基金项目
国家重点基础研究发展规划(973)项目(2009CB118705) （973）
公益性行业(农业)科研专项(201203083) （农业）
贵州省农业科技攻关“喀斯特山区大鲵模拟生境繁育配套技术研究”项目(黔科合NY字[2010]3053号)资助 （黔科合NY字[2010]3053号）
