水生生物学报Issue(3)：467-473,7.DOI:10.7541/2014.66
雌激素对中华鳖性腺分化及DMRT1、SOX9基因表达的影响
THE EFFECTS OF ESTROGEN ON GONADAL DIFFERENTIATION AND EXPRESSIONS OF DMRT1 AND SOX9 IN PELODISCUS SINENSIS
摘要
Abstract
The molecular mechanism of sex determination in Pelodiscus sinensis has been a long-standing mystery. In many vertebrate species, estrogens play a key role in sex determination and gonadal differentiation. To investigate the effect of estrogens in the gonadal differentiation of P. sinensis and the underlying molecular mechanism, we treated the undifferentiated embryos with 17β-estradiol (E2) and aromatase inhibitor (AI). Results showed that the female ratio in-creased by 92.3% after E2 treatment, whereas female ratio in AI treatment group decreased dramatically to 13.1%. Meanwhile, the obvious masculinization of genetically female embryos and feminization of genetically male embryos were observed after AI and E2 treatment, respectively. Moreover, RT-PCR and immunofluorescence analysis showed that E2 could significantly downregulated mRNA and protein expressions of DMRT1 and SOX9, which were the key factors involving in male development. However, AI exerted the opposite effect. In conclusion, estrogens inhibited the male development via the inhibition of expression of DMRT1 and SOX9, and consequently led to female development, indi-cating the important role of estrogens in the female sexual differentiation in Pelodiscus sinensis.关键词
雌激素/DMRT1/SOX9/性别分化/性腺发育/中华鳖Key words
Estrogens/DMRT1/SOX9/Sexual differentiation/Gonadal development/Pelodiscus sinensis分类
生物科学引用本文复制引用
王莉,胡涛,宓猛冬,杨克周,钱国英,葛楚天..雌激素对中华鳖性腺分化及DMRT1、SOX9基因表达的影响[J].水生生物学报,2014,(3):467-473,7.基金项目
国家自然科学基金(31101884) （31101884）
973计划前期专项(2011CB111513) （2011CB111513）
浙江省公益性技术应用研究项目(2013C32054) （2013C32054）
宁波市科技创新团队(2012B82016) （2012B82016）
宁波市自然科学基(2012A610142)资助 （2012A610142）
