水生生物学报Issue(3)：474-479,6.DOI:10.7541/2014.67
不同营养方式对小球藻FACHB 484生长的影响及其非自养生长机理研究
STUDY ON THE EFFECTS OF DIFFERENT NUTRITION MODES ON THE GROWTH OF CHLORELLA SP. FACHB 484 AND THE MECHANISM OF HETEROTROPHIC GROWTH
摘要
Abstract
Here we systematically studied the growth of Chlorella sp. FACHB 484 in glucose-containing medium in different nutrition modes. We also conducted the inhibition tests to investigate the effects of glucose on the growth of Chlorella sp. FACHB 484 in heterotrophic and mixotrophic conditions, as well as to identify the key enzyme in oxida-tion respiratory system. We observed that Chlorella sp. FACHB 484 could utilize glucose as the source of nutrition in all the test nutritional modes and showed different growth rates in the order mixotrophy > photoheterotrophy >light-activated heterotrophy > chemoheterotrophy > photoautotrophy. The maximum biomass and specific growth rate (SGR) of the tested algae in mixotrophy were 8.6 and 3.4 times higher than those in photoautotrophy. The value of SGR of Chlorella sp. FACHB 484 in mixotrophy was close to the sum of the values of SGRs in photoautotrophy and photo-heterotrophy. In mixotrophic and photoheterotrophic modes, Chlorella sp. FACHB 484 used glucose and light as the sources of carbon and energy respectively. The cells of Chlorella sp. FACHB 484 expresses succinic dehydrogenase and cytochrome oxidase which are most likely the key enzymes of the oxidation respiratory chain.关键词
小球藻/异养生长/兼养生长/代谢机理Key words
Chlorella sp./Heterotrophic growth/Mixotrophic growth/Metabolic mechanism分类
农业科技引用本文复制引用
牛海亚,马玉龙,石勋祥,牛芮..不同营养方式对小球藻FACHB 484生长的影响及其非自养生长机理研究[J].水生生物学报,2014,(3):474-479,6.基金项目
国家973计划前期研究专项(No.2012CB723106) （No.2012CB723106）
宁夏环境科学研究项目(No.20100803)资助 （No.20100803）
