水生生物学报Issue(3)：516-524,9.DOI:10.7541/2014.73
超声波对铜绿微囊藻超微结构和生理特性的影响
EFFECTS OF ULTRASOND WAVE ON THE ULTRASTRUCTURE AND PHYSIOLOGICAL CHARACTERISTICS OF BLUE-GREEN ALGAE (MICROCYSTIS AERUGINOSA)
摘要
Abstract
To study the effects of ultrasound wave on the blue-green algae (Microcystis aeruginosa), blue-green algae were exposed to ultrasound waves (40W) for 20 minutes, and then cultured for 1, 3, and 5 days. The results indicated that the biomass of suspended algae cells reduced by 97.84% at Day 3. From Day 1 to Day 3, lipid bodies and cyano-phycin granules were accumulated, and thylakoid layers were cracked, and phycobilisomes attached to thylakoid dropped off in the cells. At Day 5, the nucleoid area gradually shrinked or disappeared, the cell infrastructure disinte-grated, and voids appeared in cytoplasm and intracellular granules were degraded. Moreover, the photosynthetic oxygen evolution rate decreased by 24.83%on Day 3. Chl.a content reduced by 23.75%on Day 5. SOD activity and CAT acti-vity were induced by ultrasound, although both of the activities diminished from Day1 to Day 5. The amount of intracellular organic exudation increased after treatment. The activities of three ATPases (Na+/K+-ATPase, Mg2+-ATPase and Ca2+-ATPase) increased firstly and then decreased, which was related to the change of membrane permeability. These results suggested that ultrasound wave may reduce algae photosynthesis, damage photosynthetic pigments and increase the membrane permeability, which can cause oxidative damage and induce death of M. aeruginosa. The dy-namic CAT and ATPases activities suggested that M. aeruginosa may enhance antioxidant enzymes activity as well as ion regulation and energy activity to resist the ultrasound stress, and that the growth and metabolism of algae cells began to recover over time while the enzyme activities began to decline.关键词
超声波/铜绿微囊藻/超微结构/光合作用/抗氧化酶/ATP酶Key words
Ultrasound/Microcystis aeruginosa/Ultrastructure/Photosynthesis/Antioxidant enzyme/ATPase分类
生物科学引用本文复制引用
万莉,邵路路,陆开宏,朱津永,杨文..超声波对铜绿微囊藻超微结构和生理特性的影响[J].水生生物学报,2014,(3):516-524,9.基金项目
国家教育部高等学校博士学科点专项科研基金(20123305110001) （20123305110001）
国家自然科学基金(30771658) （30771658）
宁波市重大科技攻关项目(2008C50017) （2008C50017）
宁波大学研究生科研创新基金(G12JA029) （G12JA029）
国家自然科学基金项目(31302192) （31302192）
浙江省教育厅科研项目(Y201327177) （Y201327177）
宁波市自然科学基金项目(2013A610171)资助 （2013A610171）
