水生生物学报Issue(3)：533-539,7.DOI:10.7541/2014.75
微囊藻毒素-LR对小鼠肠道消化酶的影响
EFFECTS OF MICROCYSTIN-LR ON THE DIGESTIVE ENZYME ACTIVITY OF INTESTINAL TRACT IN BALC/C MICE
摘要
Abstract
Microcystin-LR has been known to harm the health in both humans and animals. Recently there has been an increase in reports about gastrointestinal ailments resulting from the ingestion of MCLR. Some data also indicated that MCLR could accumulate in the intestinal tract and cause injuries. In this study, we intraperitoneally injected mice with MCLR for 28 days, and observed the pathophysiological and ultrastructural changes. We also examined the activities of intestinal mucosal brush border membrane enzymes. Severe injuries were found in villi, such as a decrease in villi number and the exfoliation of villi into gut cavity. The cells of lamina propria and submucosa showed swell and hy-peremia. In the ultrathin sections, loss of density in the cytoplasm associated with swollen mitochondria as well as nu-clei deformation was observed. The activities of intestinal disaccharidase (sucrase, maltase, lactase), alkaline phos-phatase, and γ-glutamyl transferase decreased after the MCLR exposure. Based on these results, we reasoned that the digestion capacity of small intestine might be inhibited after subchronic exposure of MCLR, which led to malabsorption. Our study suggested new cellular mechanisms that explain gastrointestinal sickness, such as nausea, vomiting and diar-rhea, induced by cyanobacterial toxins.关键词
微囊藻毒素/肠道/酶活/超微结构Key words
Microcystin/Intestinal tract/Enzyme activity/Ultrastructure分类
资源环境引用本文复制引用
刘鹿忆,谢平..微囊藻毒素-LR对小鼠肠道消化酶的影响[J].水生生物学报,2014,(3):533-539,7.基金项目
中国国家自然科学基金(31070457)资助 （31070457）
