水生生物学报Issue(3)：563-570,8.DOI:10.7541/2014.79
一种新型贻贝抗菌肽的分离纯化及鉴定
A NOVEL ANTIMICROBIAL PEPTIDE IDENTIFIED FROM MYTILUS CORUSCUS
摘要
Abstract
The researches on antibacterial peptides from Mytilus coruscus, an important mytilus on aquiculture, have significant value that helping people to understand the mechanism of innate immune system of this mussel. By using multi-dimensional reverse phase high performance liquid chromatography (RP-HPLC), a novel antimicrobial peptide was isolated from Mytilus coruscus serum. The mass and the N-terminal sequence of this peptide were analyzed by a combination of Mass Spectrometry and Edman degradation. This peptide consisted 55 amino acids and had a 6621.55 D with 6 Cysteines presented in its sequence forming 3 disulfide bonds. A chitin-biding domain was detected by domain analysis and the peptide was named mytichitin-A therefore. The mRNA transcripts of mytichitin-A were mainly detected in gonad, which indicated that mytichitin-A were specifically synthesized and stored in genital system. The expression level of mytichitin-A in gonad was up-regulated and reached to the highest level at 12h after bacterial challenge, which was 9-fold increase compared to that of the control group. These results indicated that mytichitin-A was involved in the host immune response against bacterial infection and might contribute to the clearance of invading bacteria.关键词
厚壳贻贝/抗菌肽/几丁质结合结构域/荧光定量PCRKey words
Mytilus coruscus/Antibacterial peptide/Chitin-biding domain/RT-PCR分类
生物科学引用本文复制引用
孙敬敬,刘慧慧,周世权,王信超,范美华,申望,廖智..一种新型贻贝抗菌肽的分离纯化及鉴定[J].水生生物学报,2014,(3):563-570,8.基金项目
浙江省科技厅面上科研农业项目(2009C32016)资助 （2009C32016）
