水生生物学报Issue(5)：848-853,6.DOI:10.7541/2014.127
不同壳色三角帆蚌外套膜基因的SRAP-cDNA差异分析
ANALYSIS OF DIFFERENTIAL GENE EXPRESSION BY SRAP-cDNA IN MANTLE TISSUE OF HYRIOPSIS CUMINGII WITH DIFFERENT EXTERNAL SHELL COLOR
摘要
Abstract
The segregation of shell coloration and presence of radial rays in the juvenile freshwater mussel, Hyriopsis cumingii, follows Mendelian inheritance modes of a single gene locus with one pair of dominant/recessive alleles. To elucidate the mechanisms of external shell color formation, gene expression of two typical shell colors was analyzed by bulked segregant analysis (BSA) and SRAP-cDNA. Five differential gene segments were obtained by SRAP-cDNA using 30 primers’ combinations, and 14 fragments with the size ranging from 195 bp to 339 bp were obtained after re-claim and clone. Using BlastX, these fragments could encode two similar proteins, dihydropyrimidine dehydrogenase (DPD) and MYM-1 Zn Finger protein, and no protein related to pigment could be encoded. Further directions should focus on the relationship between shell color phenotype and pyrimidine metabolism by similar Zn finger proteins in the triangle pearl mussels.关键词
三角帆蚌/壳色表型/外套膜/SRAP-cDNA/差异基因Key words
Hyriopsis cumingii/Shell color phenotype/Mantle tissue/SRAP-cDNA/Differential genes分类
生物科学引用本文复制引用
闻海波,曹哲明,金武,顾若波,华丹,黄晓飞,徐跑..不同壳色三角帆蚌外套膜基因的SRAP-cDNA差异分析[J].水生生物学报,2014,(5):848-853,6.基金项目
国家公益性行业科研专项(200903028) （200903028）
中央级公益性科研院所基本科研业务专项(20l1JBFA18)资助 （20l1JBFA18）
