水生生物学报Issue(6)：1002-1007,6.DOI:10.7541/2014.148
池蝶蚌致雄性化基因fem-1c及其蛋白分子的结构特征分析
STRUCTURE CHARACTERISTICS OF A MASCULINIZED FEM-1C GENE AND ITS PROTEIN FROM HYRIOPSIS SCHLEGELII
摘要
Abstract
We first reported the masculinized feminization gene (fem-1c) in the freshwater pearl mussel Hyriopsis schlegelii, which was cloned by Race-PCR from gonad tissue. Gene structure, multiple sequence homology alignment, transmembrane region, hydrophilia and hydrophobic property, and functional domain prediction was further analyzed using bioinformatics approaches. The full length of fem-1c gene was 2328 bp that contained an open reading frame of 1869 bp encoding 622 amino acid residues. The corresponding protein was stable and hydrophilic. Multiple sequence homology alignment showed that the Fem-1c proteins from H. schlegelii and C. gigas shared the highest amino acid identity (79%). The secondary structure of fem-1c protein mainly containedα-helix and random coil forming 9 ankyrin repeat (ANK) motifs. We also found 9 ANK motifs clearly in tertiary structure prediction by SMART soft. The conser-vation of the fem-1c from invertebrates to vertebrates suggested that these genes have a common ancient origin and that they may play a conserved role in the functions of these organisms. These results suggest that fem-1c gene in Hyriopsis schlegelii may have the similar functions with nematode Caenorhabditis elegans in sex determination.关键词
池蝶蚌/fem-1c基因/克隆/序列分析/结构预测Key words
Hyriopsis schlegelii/fem-1c gene/Clone/Sequence analysis/Structure Prediction分类
生物科学引用本文复制引用
熊文芳,史建伍,蒋谋炎,陈永玲,彭扣,盛军庆,王军花,洪一江..池蝶蚌致雄性化基因fem-1c及其蛋白分子的结构特征分析[J].水生生物学报,2014,(6):1002-1007,6.基金项目
国家自然科学基金(31160534) （31160534）
江西省科技落地计划(12001) （12001）
江西省科技支撑计划项目(20112BBF60013,20121BBF 60036) （20112BBF60013,20121BBF 60036）
江西省自然科学基金(20122BAB204016) （20122BAB204016）
江西省教育厅科技项目(GJJ12020)资助 （GJJ12020）
