水生生物学报Issue(6)：1047-1053,7.DOI:10.7541/2014.154
克氏原螯虾源致病性豚鼠气单胞菌的分离及其生物学特性
ISOLATION AND BIOLOGICAL CHARACTERISTICS OF AN AEROMONAS CAVIAE PATHOGEN FROM PROCAMBARUS CLARKII
摘要
Abstract
A pathogenic strain L2M-A was isolated from diseased Procambarus clarkii, and was identified as an Aero-monas caviae strain (GenBank accession No. KF446251) with biochemical and molecular approaches. The 16S rDNA sequence of this strain showed a homology of 99%to 100%with those of Aeromonas strains, and was closest related to that of A. caviae strain JXZ-3 (GenBank accession No:JF496552). We also found that the optimal growth conditions of strain L2M-A were pH5-9, temperature at 30℃, and spinning speed at 200 r/min. The growth of L2M-A could be sig-nificantly inhibited by difloxacin at the concentration of ≥6.25 μg/mL. This finding could provide scientific guidance for the disease control.关键词
豚鼠气单胞菌/鉴定/生物学特性/克氏原螯虾Key words
Aeromonas caviae/Identification/Biological characteristics/Procambarus clarkii分类
农业科技引用本文复制引用
曹海鹏,温乐夫,杨移斌,石婷婷,何珊..克氏原螯虾源致病性豚鼠气单胞菌的分离及其生物学特性[J].水生生物学报,2014,(6):1047-1053,7.基金项目
江苏省科技支撑计划项目(No. BE2013366) （No. BE2013366）
山西省科技攻关项目(No.20120311025-4) （No.20120311025-4）
上海高校实验技术队伍建设计划(个人)项目 （个人）
上海海洋大学水产类人才创新创业实训基地(泗洪)项目 （泗洪）
国家自然科学基金青年基金项目资助 （）
