水生生物学报Issue(6)：1054-1061,8.DOI:10.7541/2014.155
斑马鱼胚胎发育过程中TGF-β1基因的表达特征分析
ANALYSIS OF THE EXPRESSION OF TGF-β1 GENE IN ZEBRAFISH EMBRYOGENESIS
摘要
Abstract
Transforming growth factor-β1 (TGF-β1) is a pleiotropic cytokine and is involved in the regulation of key events in development, disease, and tissue repair. Here we explored the expression of TGF-β1 in zebrafish of which the full cDNA consists of 1571 bp and encodes 377aa. The sequence of this gene was obtained from NCBI. Phylogenetic analysis revealed that TGF-βs were classified strictly with the same type of proteins. TGF-β1 of zebrafish was classified on the same branch of the phylogenetic tree with TGF-β1s of other fish. RT-PCR of this gene at different embryonic stages showed that TGF-β1 existed in unfertilized eggs of zebrafish. The expression started to increase at 24hpf and maintained a high level until 72hpf. Whole-mount in situ hybridization (WISH) analysis showed that TGF-β1 transcripts could be first detected in embryos at 24hpf and increased noticeably at later developmental stages. TGF-β1 transcripts were mainly expressed in gill arches, lateral line primordium, otic vesicle, olfactory placode, heart and pronephros. These indicated that TGF-β1 might play an essential role in immunoregulation, lateral line formation and vasculogenesis. Furthermore, the effect of hypoxia on the expression of TGF-β1 in zebrafish embryogenesis was evaluated with RT-PCR, Real-Time PCR and WISH. After the hypoxia treatment the development of embryos was delayed and the expression of TGF-β1 in embryos decreased dramatically. These results suggested that TGF-β1 played an important role in zebrafish embryogenesis and might be related to the hypoxia-induced delay in zebrafish embryonic developement.关键词
斑马鱼/TGF-β1基因/胚胎发育/低氧Key words
Zebrafish/TGF-β1/Embryogenesis/Hypoxia分类
生物科学引用本文复制引用
侯少丰,李艳丽,徐功玉,赵浩斌,周青春,钟雪萍..斑马鱼胚胎发育过程中TGF-β1基因的表达特征分析[J].水生生物学报,2014,(6):1054-1061,8.基金项目
国家自然科学基金项目(30972254,31272645) （30972254,31272645）
中央高校基本科研业务费专项资金科研项目(CCNU12A02007)资助 （CCNU12A02007）
