水生生物学报Issue(1)：101-109,9.DOI:10.7541/2015.13
团头鲂生长相关基因在不同发育时期生长轴组织的表达分析
THE EXPRESSION ANALYSIS OF GROWTH-RELATED GENES IN GROWTH-RELATED TISSUES IN DIFFERENTIAL GROWTH GROUPS AT DIFFERENT DEVELEPMENTAL STAGES OF MEGALOBRAMA AMBLYCEPHALA
摘要
Abstract
In this study, we investigated the expression of growth-related genes in differential growth groups at different developmental stages of M. amblycephala. We employed real-time PCR to quantify the expression of six growth-related genes in the brain, the liver and muscles. The results revealed that GHRs genes were highly expressed in the liver and muscles, and their expression level in 6-month and 12-month-old fish was higher than that in 3-month-old fish, and the expression level in the fast-growing group was higher than that in the slow-growing group (P<0.05). IGFs genes were expressed in all three tissues, and the expression in the liver was the highest. The expression of IGFs decreased as the fish grew older (P<0.05). MSTN a was primarily expressed in the brain and the liver. Unlike MSTN a, MSTN b was highly expressed in the muscles. Moreover, the expression levels of MSTN genes were negatively correlated with the growth rates (P<0.05). The Cluster analysis showed that except for MSTN a, the expression levels of the other five genes in the differential growth groups could be clustered. The cluster results revealed that the expression patterns were simi-lar in different growth-related tissues at different stages, except in the liver at 3 month and the muscles at 12 month. A positive correlation between the expression levels of MSTN a and GHR 2 was observed in the present study with Pearson correlation analysis. The similar correlation was also observed between MSTN a and IGFs. Furthermore, there was a significant positive correlation between the two differential growth groups in terms of the expression of the growth-related genes (P<0.01).关键词
团头鲂/生长基因/表达分析/聚类Key words
Megalobrama amblycephala/Growth related genes/Expression analysis/Clustering analysis分类
生物科学引用本文复制引用
易少奎,高泽霞,赵鸿昊,赵玉华,王卫民..团头鲂生长相关基因在不同发育时期生长轴组织的表达分析[J].水生生物学报,2015,(1):101-109,9.基金项目
现代农业产业技术体系建设专项(CARS-46-05) （CARS-46-05）
国家自然科学基金(31201988) （31201988）
湖北省国际合作项目(No.2013BHE006) （No.2013BHE006）
中央高校基本科研业务费专项资金(2013PY066,2013PY075和2014PY013) （2013PY066,2013PY075和2014PY013）
武汉市武昌鱼繁育工程技术研究中心建设项目(2013020705010318)资助 （2013020705010318）
