水生生物学报Issue(1)：155-163,9.DOI:10.7541/2015.20
基于叶绿体psaA和psbA基因的中国熊野藻属植物系统发育分析
PHYLOGENETIC ANALYSIS OF GENUS KUMANOA BASED ON CHLOROPLAST PSAA AND PSBA GENES
摘要
Abstract
Kumanoa intorta (former Batrachospermum intortum) and K. curvata (former B. curvatum) are two species of genus Kumanoa originally from China. In this study we amplified and sequenced the chloroplast psaA and psbA genes of these two species and of other six freshwater Rhodophyta species. We aligned their sequences with related groups from GenBank, and constructed phylogenetic trees of both single gene and combined genes by using the methods of Bayesian analysis, maximum likelihood and neighbor-joining. The trees generated with three different methods showed similar topologies and thus inferred consistent phylogenetic relationships. The two species from genus Kumanoa could form one clade with high supportive values but should be separated from genus Batrachospermum. The analysis above supported our report about a new genus Kumanoa. The molecular biological study of Kumanoa species originally from China revealed similar results with those originally from South America and Australia, indicating wide geographi-cal areas of this genus. The phylogenetic tree demonstrated a close relationship between genus Kumanoa and Batra-chospermum. According to the characteristics of the carpogonial branch, we deducted that the evolution of genus Ku-manoa was later than that of Batrachospermum, but earlier than that of Acrochaetiales and Thoreales. B. gelatinosum should be separated from other section Batrachospermum species and be classified as an independent section. The con-struction of the order Thoreales could be supported by the fact that Thoreales and Batrachospermales should be sepa-rated. Furthermore, our study suggested that psaA and psbA genes could be used as molecular markers in the study of the phylogenetic relationships of Rhodophyta.关键词
熊野藻属/psaA/psbA/系统发育Key words
Kumanoa/psaA/psbA/Phylogenetic analysis分类
农业科技引用本文复制引用
南芳茹,冯佳,谢树莲..基于叶绿体psaA和psbA基因的中国熊野藻属植物系统发育分析[J].水生生物学报,2015,(1):155-163,9.基金项目
国家自然科学基金项目(31200164,31370239)资助 （31200164,31370239）
