水生态学杂志Issue(6)：99-105,7.
微生态制剂对匙吻鲟生长性能、体组成及血清生化指标的影响
Effect of Probiotics on Growth Rate, Body Composition and the Serum Chemistry of Paddlefish Polyodon spathula
摘要
Abstract
Previous studies have demonstrated that dietary supplementation with probiotics in aquaculture helps in-crease the feed conversion ratio, improves growth rate and body composition, enhances the immune ability and health of fish.Polyodon spathula, is an ancient fresh water fish, introduced to China in 1988 and characterized by fast growth and adaptability.Until now, reports on Polyodon spathula were primarily about biology, reproduction and culturing technology, digestive enzyme research and evaluation of meat quality.The present study attempts to understand the effect of probiotics on growth rate, body composition and serum chemistry of Polyodon spathula.A total of 400 paddlefishes were randomly allotted to two groups and reared in two net cages (6 m ×6 m ×3 m) .The control group was fed a basal diet consisting of 47.8% crude protein, 9.7% moisture, 3.6% crude lipid and 10.6%crude ash and the treatment group was fed the basal diet supplemented with Probiotics A, containing Lacto-bacillus, Saccharomyces, Photosynthetic bacteria, Cusuanjun, Bacillus natto and Actinobacteria (7.47 ×107 cfu/g, 5 mL/kg) and Probiotics B containing ulfolobus acidocaldarius, Streptococcus faecium and Photosynthetic bacteria (2.23 ×108 cfu/g, 5 mL/kg).The experiment lasted 80 days, from Aug.1 to Oct.20 of 2012.During the feed-ing trial, 50 paddlefishes were randomly selected and the weight, body length and full length were recorded every 20 days.The data of final weight, relative weight of visceral mass and hepatopancrease, intestine length , body length, full length and number of paddlefishes were recorded after the fish had gone without feeding for 24 h.The body composition of muscle and liver and the serum biochemical parameters of Polyodon spathula were determined at the end of the feeding trial.Results indicate a significant difference in final weight ( treatment, 296.89 ± 86.17 g;control, 275.03 ±85.14 g)(P<0.05) and a highly significant difference (P<0.01) in the both body length(treatment, 24.55 ±2.22 cm;control, 23.55 ±2.33 cm) and full length (treatment, 47.35 ±3.54 cm;control, 46.16 ±3.87 cm) .The feed coefficient of the treatment group (2.02) was lower than that of the control group (2.11) and the relative rate of weight gain and the specific growth rate of the treatment group were, respec-tively, 11.24%and 5.56%higher than the control group.The viscerosomatic index, hepatosomatic index, the ra-tio of intestine length to body length and the crude protein content, crude fat content, crude ash content and mois-ture contents of the muscle and liver were not significantly different between the two groups (P>0.05).Measure-ment of serum biochemical parameters for Polyodon spathula reveal significantly lower levels ( P<0 .05 ) of gluta-mate pyruvate transaminase (ALT), (treatment, 200.14 ±35.85 U/mL;control, 253.80 ±23.72 U/mL) andγ-glutamyltranspeptidase (γ-GGT), (Treatment, 0.76 ±0.36 U/mL;control, 1.33 ±0.32 U/mL).Serum levels of glutamic oxalacetic transaminase (AST), total protein (TP), albumin(ALB), globulin (GLB), ALB/GLB, glucose ( GLU) , total cholesterol ( CHOL) , triglyceride ( TG) , high-density lipoprotein ( HDL-c) and low density lipoprotein ( LDL-c) were not significantly different between groups.It can be concluded that dietary supplementa-tion with probiotics increases growth rate, enhances feed utilization and improves the health of Polyodon spathula. The results of this investigation provide data to extend probiotics theory and provide practical scientific knowledge to enhance culturing of Polyodon spathula.关键词
微生态制剂/匙吻鲟/生长性能/体组成/血清生化指标Key words
probiotics/polyodon spathula/growth performance/body composition/serum biochemical parameters分类
生物科学引用本文复制引用
方程,马明洋,吉红,单世涛..微生态制剂对匙吻鲟生长性能、体组成及血清生化指标的影响[J].水生态学杂志,2014,(6):99-105,7.基金项目
西北农林科技大学安康水产试验示范站建设项目，陕西省科技厅科技型中小企业技术创新项目 （）
