水生态学杂志Issue(2)：95-100,6.DOI:10.15928/j.1674-3075.2015.02.015
复合益生菌对中华鳖生长及血液生化指标的影响
Effects of Compound Probiotics on the Growth and Blood Biochemistry of Trionyx sinensis
摘要
Abstract
Industrial aquaculture of Trionys sinensis has developed rapidly due to the high nutrient content and eco-nomic value of the species,but it has also led to a higher incidence of disease.The abuse of antibiotics on Trionys sinensis creates problems,such as bacterial resistance and drug residues,that negatively affect the healthy develop-ment of this aquaculture sector.Probiotics,as a new dietary supplement that are non-toxic,don′t lead to resistance and leave no residue could,to some extent,replace antibiotics.The aim of this investigation was to evaluate the effects of compound probiotic dose on the growth rate and blood biochemistry of Trionys sinensis so as to use com-pound probiotics more effectively and provide a theoretical basis for the healthy culturing of Trionys sinensis.The probiotics used were compounded in our laboratory and we have applied for a patent (number 201410039520.3). The probiotic components include bacillus subtilis,bacillus natto,Lactobacillus casei and Candida utilis.Test ani-mals of mean initial weight (227.47 ±14.42)g were selected for the 140 d experiment and fed a soft-shelled turtle diet three times a day supplemented with different levels of compound probiotics (Group I:0 mg/kg;Group II:500 mg/kg;Group III:1 000 mg/kg;Group IV:2 000 mg/kg).Each group consisted of 1200 individuals and each group was tested in triplicate.The water temperature,food consumption and mortality were recorded each day.After the trial was complete,15 individuals from each group were selected randomly to evaluate digestive en-zyme activity in the intestine and 10 individuals for each group were selected and fasted 24 h to measure blood bio-chemical indices including total protein (TP),total cholesterol (CHO),triglyceride (TG),glucose (GLU), blood urea nitrogen (BUN),globulin (GLB),albumin (ALB),lysozyme (LZM),methane dicarboxylic aldehyde (MDA),superoxide dismutase (SOD),glutathione peroxidase (GSH-PX),glutamic oxalacetic transaminase (AST)and alanine transaminase (ALT).Results indicate that weight gain rate (WGR),specific growth rate (SGR)and survival rate increased significantly and the bait coefficient decreased significantly compared to the con-trol group (P <0.05).The highest weight gain (29.25%),specific growth rate and survival rate as well as the lowest bait coefficient was displayed by Group III.The activities of lipase,amylase,tryptase increased significantly with the addition of compound probiotics (P <0.05),but the activity of amylase significantly decreased in Group IV with a 2 000 mg/kg compound probiotic supplement.The contents of CHO,BUN and the activity of ALT in ser-um significantly decreased (P <0.05),and the level of TG significantly increased (P <0.05),while the content of TP,GLB,ALB,GLU,MDA and the activity of AST in serum among all experimental groups showed no signifi-cant difference (P >0.05).The activities of LZM,GSH-PX and SOD in serum significantly increased (P <0.05) in Group Ⅱ and Group Ⅲ,but activities declined in Group IV (P >0.05).Based on these findings,we conclude that properly compounded probiotic supplementation to the basal diet of Trionyx sinensis can improve growth,in-crease digestive enzyme activity,enhance nonspecific immunity and antioxidant capacity and decrease the bait coef-ficient.The appropriate level of compound probiotics added in the basal diet is 1 000 -2 000 mg/kg.关键词
中华鳖/复合益生菌/生长/血液生化指标Key words
Trionyx sinensis/compound probiotics/growth/blood biochemical indices分类
生物科学引用本文复制引用
桂琳,王晓清,康银,曾丹,蒋迪,廖学敏..复合益生菌对中华鳖生长及血液生化指标的影响[J].水生态学杂志,2015,(2):95-100,6.基金项目
国家自然科学基金项目（31172406）；湖南省教育厅科学研究重点项目（11A051）。 （）
