水生态学杂志Issue(3)：18-24,7.DOI:10.15928/j.1674-3075.2015.03.003
杭州西湖底泥反硝化作用初探
Preliminary Study of Denitrification in the Sediments of West Lake, Hangzhou
摘要
Abstract
Denitrification is one of the effective ways of removing nitrate and it occurs primarily in sediments .Re-search shows that the intensity of denitrification affects overall nitrogen removal from lakes , and physical and chemi-cal factors in the water body and sediments influence the rate of denitrification .Therefore submerged plants can in-directly influence denitrification by altering physical and chemical parameters .In this study , sediment denitrifica-tion and factors influencing denitrification were investigated in West Lake , a lake in Hangzhou with high NO 3--N. The denitrification rate was represented by nitrate reductase activity and denitrification intensity , which were meas-ured and then related to factors that can affect denitrification .In December , 2013 ( winter ) and June , 2014 ( sum-mer ) , the investigation was carried out in five areas of the lake and three lake bays that included 13 sampling sites . Water samples were collected 5-10 cm below the surface for determination of water quality parameters including NH4+-N,NO2--N,NO3--N,TN and TP.Water DO, temperature and pH were determined in situ using an ORION 5-Star water quality analyzer .Sediment samples were collected using a sediment corer for determination of total or -ganic carbon (TOC), nitrate reductase activity (Nr) and denitrification intensity (De).Concentration ranges of total nitrogen (TN) in winter and summer were 1.568-2.613 mg/L and 1.117-2.848 mg/L, respectively, and the average ratios of nitrate ( NO3--N) to total nitrogen ( TN) were 76%and 72%.The ranges of sediment denitri-fication intensity in winter and summer were 0.132-1.350 mg/g and 0.643-1.286 mg/g and for nitrate reduc-tase activity, ranges were 0.015-5.092 μg/g and 0.665-19.123 μg/g.Nitrate reductase activity displayed a highly significant difference between winter and summer ( P<0.01 ) , while denitrification intensity in winter and summer were not significantly different (P>0.05).In summer, there was a highly significant positive correlation of denitrification intensity with temperature (P<0.01) and a negative correlation with NH4+-N (P<0.05).Ni-trate reductase activity was positively correlated with TOC in sediment (P<0.05) and, in winter, the denitrifica-tion intensity displayed a highly significant positive correlation with nitrate reductase activity ( P<0 .01 ) and a sig-nificant positive correlation with surface water DO ( P<0.05 ) .Our study also found that submerged plants in a certain range of coverage increased the rate of denitrification , while excessive coverage inhibited denitrification . These results provide basic data that can be used to select treatments for improving water quality in West Lake .关键词
杭州西湖/底泥/反硝化强度/硝酸盐还原酶活性Key words
West Lake , Hangzhou/sediment/denitrification rate/nitrate reductase activity分类
资源环境引用本文复制引用
张丹,王川,王艳云,周巧红,吴振斌..杭州西湖底泥反硝化作用初探[J].水生态学杂志,2015,(3):18-24,7.基金项目
国家“十二·五”水专项（2012ZX07101007-005）；国家自然科学基金（31123001；51208498；51308530）。 （）
