中国生态农业学报Issue(2)：233-238,6.DOI:10.13930/j.cnki.cjea.140853
看麦娘根系对小麦根水提液化感作用的生理响应
Physiological response of Alopecurus aequalis root to allelopathic effects of wheat (Triticum aestivum L.) root tissue extracts
摘要
Abstract
In order to illustrate the mechanism of wheat allelopathy, two strong allelopathic wheat accessions (‘115/Qinghai’ and‘92L89’) and one weak allelopathic accession (‘Kang10103’) were used in a set of hydroponic culture experiments. Roots of Alopecurus aequalis were treated with 0%, 0.2%, 1.0% and 5.0% (v/v) water-based extracts of root tissues of different wheat accessions to determine physiological responses of A. aequalis root to the wheat root extracts. The results showed that extracts significantly inhibited the growth of A. aequalis, with root fresh weight decreasing with increasing extract concentration. Also strong allelopathic accessions had higher inhibitory rates than weak accession. No differences existed among the 3 accessions when extract concentration reached 5.0%. After exposure to extracts, A. aequalis root vigor declined at inhibitory rates of 52.0%−59.6%, 46.5%−55.0%and 27.2%−44.7%respectively for‘115/Qinghai’,‘92L89’ and‘Kang10103’. Also no differences were noted between the two strong allelopathic accessions. The contents of soluble protein and methane dicarboxylic aldehyde (MDA) as well as the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of A. aequalis roots increased significantly after treated with wheat root extracts. The order of the activities of SOD and POD was‘115/Qinghai’>‘92L89’≈‘Kang10103’. The activity of CAT changed significantly with changing concentrations of the extracts, and not with different wheat varieties. The contents of MDA in roots of A. aequalis treated with‘115/Qinghai’,‘92L89’ and ‘Kang10103’ root tissues extracts were respectively 10.9−25.5 times, 5.9−24.2 times and 1.2−6.8 times that of the control. Lipid peroxidation was activated and anti-oxidative flavonoids and phenols were induced in the roots by wheat allelopathy. Thus decreasing root vigor, enhancing protective enzyme activities and synthesizing anti-oxidative substances were promising physiological responses of roots of A. aequalis exposed to wheat allelopathy.关键词
看麦娘/小麦/化感作用/生理响应/根水提液Key words
Alopecurus aequalis/Wheat (Triticum aestivum L.)/Allelopathy/Physiological response/Root extract分类
农业科技引用本文复制引用
邱秋金,谢惠玲,李圆萍,王微,陈珊,肖清铁,郑新宇,林瑞余,林文雄..看麦娘根系对小麦根水提液化感作用的生理响应[J].中国生态农业学报,2015,(2):233-238,6.基金项目
国家自然科学基金项目(30671220,31070403)和福建省高校服务海西建设重点项目(0B08B005)资助 （30671220,31070403）
