中国生态农业学报Issue(4)：441-446,6.DOI:10.13930/j.cnki.cjea.141117
高温胁迫对淮阴苜蓿差异蛋白表达及生理的影响
Effects of high temperature stress on differentially expressed proteins and physiological characteristics of alfalfa
摘要
Abstract
The Medicago sativa L. cv. Huaiyin variety of alfalfa was used to determine the physical changes and related differences in protein expressions of alfalfa in response to heat stress. Electrical conductivity, malonaldehyde content and cell activity were measured in alfalfa seedlings at 25 ℃, 30 ℃, 35 ℃ and 40 ℃ in combination with different durations of heat stress (0 h, 0.5 h, 1 h, 4 h, 12 h and 24 h). In addition, alfalfa seedlings treated at 35 ℃ and 40 ℃ for 24 h were used to analyze differentially expressed protein. The results showed that electrical conductivity of the test samples increased sharply after treatment at 35 ℃ and 40 ℃ for 0.5 h, but declined for treatment durations exceeding 1 h. However, malonaldehyde content of the samples increased for treatment durations exceeding 1 h but gradually declined after 4 h. Cell activity of test samples declined with increasing treatment duration. Screening analysis of the differences in proteins showed 27 significantly different spots after treatment at 35 ℃ for 24 h, 19 of which spots were raised and 5 were new. After treatment at 40 ℃ for 24 h, 51 significantly different spots were observed—40 out of the 51 spots were raised and 11 were new. Secondary mass spectrum analyses indicated that 4 proteins — 20 kD chaperonin, Hsp70, Hsp23 and Hsp17, were only observed in samples at 40 ℃ for 24 hours. This suggested that the 4 proteins correlated with heat stress defense mechanisms, the samples produced more defense proteins at 40 ℃ to repair damage to alfalfa seedling membranes.关键词
淮阴苜蓿/高温胁迫/生理指标/蛋白质组/二级质谱/热激蛋白Key words
Medicago sativa L.cv. Huaiyin/High temperature stress/Physiological index/Proteome/Secondary mass spectrum/Heat shock protein分类
农业科技引用本文复制引用
王仪明,李伟民,雷艳芳,魏臻武,韩正晟..高温胁迫对淮阴苜蓿差异蛋白表达及生理的影响[J].中国生态农业学报,2015,(4):441-446,6.基金项目
国家自然科学基金项目(30972136)资助 （30972136）
