生物加工过程Issue(3)：37-41,5.DOI:10.3969/j.issn.1672-3678.2014.03.007
海泡石吸附固定化猪胰脂肪酶
Porcine pancreatic lipase immobilized on sepiolite
摘要
Abstract
The porcine pancreatic lipase(PPL) was immobilized on sepiolite by physical absorption. Results showed that the adsorbed maximum activity ( 309 U/g ) was observed under the conditions of immobilization time of 4 h,reactive phosphate solution of pH 6�0,and reaction temperature 25 ℃. The immobilized PPL showed excellent adaptability at higher pH and better thermal stability compared with free PPL�Meanwhile, Fourier transform infrared spectrometer ( FT-IR ) , scanning electron microscopy ( SEM) were used to analyze experiment results. Results confirmed the effects of sepiolite in experiments of lipase immobilization�关键词
固定化/猪胰脂肪酶/海泡石Key words
immobilization/porcine pancreatic lipase/sepiolite分类
生物科学引用本文复制引用
王欢,胡集铖,李亚,韩萍芳..海泡石吸附固定化猪胰脂肪酶[J].生物加工过程,2014,(3):37-41,5.基金项目
国家重点基础研究发展计划（973计划）（2009CB72470）；江苏省普通高校研究生科研创新计划 （）
