生物加工过程Issue(3)：42-46,5.DOI:10.3969/j.issn.1672-3678.2014.03.008
功能化离子液体化学修饰猪胰脂肪酶的催化性能
Chemical modification of porcine pancreatic lipase by functional ionic liquid
摘要
Abstract
Porcine pancreatic lipase ( PPL ) was modified with functionalized ionic liquids ([HOOCMMIm][Cl],and [HOOCEMIm][Cl],[HOOCBMIm][Cl])�Activity in buffer and organic solvents,thermostability,and ultraviolet spectrum of the modified PPL were compared with those of the unmodified PPL in the triacetin hydrolysis reaction�The results showed that modified enzymes were more tolerant to temperature and low pH changes and had both higher activity and stability�Functionalized ionic liquids modification enhanced thermal stability of PPL�Enzymes contained 100% activity of PPL in high concentration of methanol and N,N-dimethylformamide( DMF) after modification�After PPL was modified by functionalized ionic liquids,red shift of PPL characteristic ultraviolet peak and absorbance increase occurred due to changes of lipase microenvironment�关键词
酶/离子液体/化学修饰/猪胰脂肪酶Key words
enzyme/ionic liquid/modification/porcine pancreas lipase分类
生物科学引用本文复制引用
初旭明,贾儒,杨姣,黄和,胡燚..功能化离子液体化学修饰猪胰脂肪酶的催化性能[J].生物加工过程,2014,(3):42-46,5.基金项目
国家高技术研究发展计划（863计划）（2011AA02A209）；国家杰出青年科学基金（21225626）；国家自然科学基金青年基金（20906049）；国家自然科学基金重点项目 （）
